Savolainen H, Lehtonen E, Vainio H
Acta Neuropathol. 1977 Mar 31;37(3):219-23. doi: 10.1007/BF00686882.
The binding of CS2 sulphur to rat spinal cord neurofilaments was studied 3 and 6 h after the intraperitoneal injection of 1.3 mmol of CS2 in control and phenobarbitone pretreated rats. The binding of CS2 carbon was studied similarly 3 h after the injection. The binding of CS2 sulphur was 31% higher in the control neurofilaments than that in the phenobarbitone pretreated group. Half of the neurofilament associated CS2 sulphur was bound to protein in both groups as studied by electrophoresis. Approximately 30% of the neurofilament associated CS2 sulphur was detected 6 h after the injection of that at 3 h in both groups. All of the neurofilament associated CS2 carbon was bound to protein in control group 3 h after the injection. The binding of CS2 carbon was 4.7 times less than that of sulphur at the same time. The present data may indicate that the high binding of CS2 to the neurofilament protein contributes to the increase in neurofilaments in chronic CS2 intoxication.
在对照大鼠和经苯巴比妥预处理的大鼠腹腔注射1.3 mmol二硫化碳（CS2）后3小时和6小时，研究了CS2硫与大鼠脊髓神经丝的结合情况。在注射后3小时，同样研究了CS2碳的结合情况。对照神经丝中CS2硫的结合量比苯巴比妥预处理组高31%。通过电泳研究发现，两组中与神经丝相关的CS2硫有一半与蛋白质结合。在两组中，注射后6小时检测到的与神经丝相关的CS2硫约为3小时时的30%。注射后3小时，对照组中所有与神经丝相关的CS2碳都与蛋白质结合。此时，CS2碳的结合量比硫少4.7倍。目前的数据可能表明，CS2与神经丝蛋白的高结合导致了慢性CS2中毒时神经丝数量的增加。
