Savolainen H, Järvisalo J, Elovaara E, Vainio H
Toxicology. 1977 Apr;7(2):207-14. doi: 10.1016/0300-483x(77)90066-x.
The binding of 35S- and 14C-labelled CS2 in rat central nervous system (CNS) was studied in control and phenobarbitone-pretreated rats in vivo and in vitro. Animals received CS2 through intraperitoneal injection in olive oil. Samples were taken for analysis 3 and 6 h after the injection. Sulphur atoms were bound to rat brain more highly than carbon atoms in control and phenobarbitone-pretreated rats in vivo. The phenobarbitone pretreatment increased the cerebral binding of sulphur and decreased that of carbon. Main part of the bound sulphur and carbon was detected in the trichloroacetic acid (TCA) precipitable fraction in both test groups. Pretreatment with phenobarbitone or with polychlorinated hydrocarbon (PCB) mixture did not increase significantly the binding of CS2 sulphur in brain microsomes in vitro. The present findings suggest that a considerable amount of injected CS2 is retained in the nervous system and that phenobarbitone pretreatment of test subjects may also alter brain metabolism of CS2.
在体内和体外，对对照组大鼠以及经苯巴比妥预处理的大鼠，研究了35S和14C标记的二硫化碳（CS2）在大鼠中枢神经系统（CNS）中的结合情况。动物通过腹腔注射溶于橄榄油的CS2给药。在注射后3小时和6小时取样进行分析。在体内，对照组和经苯巴比妥预处理的大鼠中，硫原子与大鼠脑的结合程度高于碳原子。苯巴比妥预处理增加了硫在脑中的结合，降低了碳的结合。在两个试验组中，结合的硫和碳的主要部分都在三氯乙酸（TCA）可沉淀部分中检测到。在体外，用苯巴比妥或多氯代烃（PCB）混合物预处理并没有显著增加CS2硫在脑微粒体中的结合。目前的研究结果表明，注入的大量CS2保留在神经系统中，并且对试验对象进行苯巴比妥预处理也可能改变CS2的脑代谢。
