Savolainen H, Järvisalo J
Chem Biol Interact. 1977 Apr;17(1):51-9. doi: 10.1016/0009-2797(77)90071-0.
The effect of acute CS2 exposure on the rat brain protein metabolism was studied with control and phenobarbitone pretreated adult male rats 1, 4 and 46 h after exposure. Increased activity of acid proteinase was detected in both test groups 1 and 4 h after exposure and it was accompanied by changes in 14C-labelled leucine turnover as well as in RNA content. The changes were more conspicuous in cerebellum than in brain in both test groups while phenobarbitone pretreatment modified the brain response towards intoxication. This modification probably represents inherent effects of barbiturate on brain protein metabolism as well as altered metabolism of CS2. The activities of creatine kinase and nonspecific cholinesterase displayed only subtle changes as assayed in cerebral homogenate and serum. Thus a single acute CS2 intoxication apparently causes definitive transient changes in brain protein metabolism; serum enzyme determinations may not reflect the magnitude of these changes.
用成年雄性大鼠作为对照，对经苯巴比妥预处理的大鼠在接触二硫化碳（CS2）1小时、4小时和46小时后，研究急性接触CS2对大鼠脑蛋白质代谢的影响。在接触后1小时和4小时，两个试验组均检测到酸性蛋白酶活性增加，同时伴有14C标记的亮氨酸周转率以及RNA含量的变化。在两个试验组中，小脑的变化比大脑更明显，而苯巴比妥预处理改变了大脑对中毒的反应。这种改变可能代表了巴比妥酸盐对脑蛋白质代谢的固有影响以及CS2代谢的改变。在脑匀浆和血清中检测到的肌酸激酶和非特异性胆碱酯酶的活性仅显示出细微变化。因此，单次急性CS2中毒显然会导致脑蛋白质代谢发生明确的短暂变化；血清酶测定可能无法反映这些变化的程度。
