Mills G A, Walker V, Mughal H
School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK.
J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):281-5. doi: 10.1016/s0378-4347(98)00542-8.
Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography-mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen-polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories.
三甲胺尿症（鱼腥味综合征）通过三甲胺尿排泄增加且氧化三甲胺减少来诊断。我们报告了一种新的定量稳定同位素稀释气相色谱 - 质谱法，用于使用固相微萃取（SPME）分析这些代谢物。发现聚二甲基硅氧烷和混合的碳分子筛 - 聚二甲基硅氧烷SPME纤维都适用于三甲胺的顶空萃取。这种新的采样技术在代谢筛查实验室分析挥发性和半挥发性化合物方面可能有广泛应用。
