双重感染和重组感染：巴西HIV-1流行的一个组成部分。
Dual and recombinant infections: an integral part of the HIV-1 epidemic in Brazil.
作者信息
Ramos A, Tanuri A, Schechter M, Rayfield M A, Hu D J, Cabral M C, Bandea C I, Baggs J, Pieniazek D
机构信息
Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
出版信息
Emerg Infect Dis. 1999 Jan-Feb;5(1):65-74. doi: 10.3201/eid0501.990108.
We systematically evaluated multiple and recombinant infections in an HIV-infected population selected for vaccine trials. Seventy-nine HIV-1 infected persons in a clinical cohort study in Rio de Janeiro, Brazil, were evaluated for 1 year. A combination of molecular screening assays and DNA sequencing showed 3 dual infections (3.8%), 6 recombinant infections (7.6%), and 70 (88.6%) infections involving single viral subtypes. In the three dual infections, we identified HIV-1 subtypes F and B, F and D, and B and D; in contrast, the single and recombinant infections involved only HIV-1 subtypes B and F. The recombinants had five distinct B/F mosaic patterns: Bgag-p17/Bgag-p24/Fpol/Benv, Fgag-p17/Bgag-p24/Fpol/Fenv, Bgag-p17/B-Fgag-p24/Fpol/Fenv, Bgag-p17/B-Fgag-p24/Fpol/Benv, and Fgag-p17/B-Fgag-p24/Fpol/Fenv. No association was found between dual or recombinant infections and demographic or clinical variables. These findings indicate that dual and recombinant infections are emerging as an integral part of the HIV/AIDS epidemic in Brazil and emphasize the heterogenous character of epidemics emerging in countries where multiple viral subtypes coexist.
我们系统评估了入选疫苗试验的HIV感染人群中的多重感染和重组感染情况。对巴西里约热内卢一项临床队列研究中的79名HIV-1感染者进行了为期1年的评估。分子筛查检测和DNA测序相结合的方法显示，有3例双重感染（3.8%）、6例重组感染（7.6%）以及70例（88.6%）感染涉及单一病毒亚型。在3例双重感染中，我们鉴定出HIV-1亚型F和B、F和D以及B和D；相比之下，单一感染和重组感染仅涉及HIV-1亚型B和F。重组体有5种不同的B/F镶嵌模式：Bgag-p17/Bgag-p24/Fpol/Benv、Fgag-p17/Bgag-p24/Fpol/Fenv、Bgag-p17/B-Fgag-p24/Fpol/Fenv、Bgag-p17/B-Fgag-p24/Fpol/Benv以及Fgag-p17/B-Fgag-p24/Fpol/Fenv。未发现双重感染或重组感染与人口统计学或临床变量之间存在关联。这些发现表明，双重感染和重组感染正在成为巴西HIV/AIDS流行的一个组成部分，并强调了在多种病毒亚型共存国家中出现的流行的异质性特征。
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