Sasaki A, Kawano K, Aramaki M, Nakashima K, Yoshida T, Kitano S
Department of Surgery I, Oita Medical University, Japan.
J Surg Oncol. 1999 Feb;70(2):103-8. doi: 10.1002/(sici)1096-9098(199902)70:2<103::aid-jso8>3.0.co;2-h.
This study was designed to identify a difference in immunostaining that might help to distinguish between primary and metastatic liver neoplasms.
We examined immunohistochemical expression of cytokeratins (CKs) 7, 8, 19, and 20 in 12 intrahepatic cholangiocarcinomas (ICCs; 9 of the mass-forming and 3 of the infiltrating type), 25 metastatic colorectal carcinomas (MCCs), and 7 metastatic gastric carcinomas (MGCs) of the liver.
CKs 7 and 19 were expressed in all ICCs of infiltrating type, while each was seen in 7/9 (77.8%) of mass-forming type. CK 7-positive/CK 20-negative was seen in 9/12 (75.0%) of ICCs and in none of the 25 MCCs, while CK 7-negative/CK 20-positive was seen in 1/12 (8.3%) of ICCs and 20/25 (80.0%) of MCCs. No differences were observed between MGCs and ICCs.
These results suggest that immunohistochemical staining for both CKs 7 and 20 is useful for the differential diagnosis of ICCs and MCCs, whereas phenotypic expression of CKs appears to be different between mass-forming and infiltrating types of ICCs.
本研究旨在确定免疫染色方面的差异，以有助于区分原发性和转移性肝脏肿瘤。
我们检测了细胞角蛋白（CK）7、8、19和20在12例肝内胆管癌（ICC；9例肿块型和3例浸润型）、25例转移性结直肠癌（MCC）和7例肝脏转移性胃癌（MGC）中的免疫组化表达。
CK7和CK19在所有浸润型ICC中均有表达，而在肿块型ICC中分别为7/9（77.8%）。CK7阳性/CK20阴性在9/12（75.0%）的ICC中可见，而在25例MCC中均未见到；CK7阴性/CK20阳性在1/12（8.3%）的ICC和20/25（80.0%）的MCC中可见。MGC和ICC之间未观察到差异。
这些结果表明，CK7和CK20的免疫组化染色对ICC和MCC的鉴别诊断有用，而肿块型和浸润型ICC中CK的表型表达似乎有所不同。
