一种化脓隐秘杆菌（放线菌）突变体，其形成孔道的细胞溶素化脓素的产生存在缺陷，毒力降低。
An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence.
作者信息
Jost B H, Songer J G, Billington S J
机构信息
Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona 85721, USA.
出版信息
Infect Immun. 1999 Apr;67(4):1723-8. doi: 10.1128/IAI.67.4.1723-1728.1999.
Abstract
Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an approximately 1. 8-log10 reduction in the 50% infectious dose compared to that for wild-type A. pyogenes in a mouse intraperitoneal infection model. Studies involving cochallenge of wild-type and PLO-1 bacteria resulted in recovery of similar numbers of both strains, suggesting that PLO production is required for survival in vivo. Recombinant, His-tagged PLO (His-PLO) is cytotoxic for mouse peritoneal macrophages and J774 cells in a dose-dependent manner. Protection against challenge with A. pyogenes could be afforded by vaccination with formalin-inactivated His-PLO, suggesting that PLO is a host-protective antigen, as well as a virulence determinant.
摘要
化脓隐秘杆菌（放线菌属）产生的溶血外毒素——化脓隐秘杆菌溶素（PLO），是细菌毒素硫醇激活溶细胞素家族的成员。plo基因的插入失活导致PLO表达缺失，同时溶血活性丧失。在小鼠腹腔感染模型中，plo突变体PLO-1的50%感染剂量与野生型化脓隐秘杆菌相比，降低了约1.8个对数10。涉及野生型和PLO-1细菌共同攻击的研究结果显示，两种菌株的恢复数量相似，这表明产生PLO是在体内存活所必需的。重组的、带有His标签的PLO（His-PLO）对小鼠腹腔巨噬细胞和J774细胞具有剂量依赖性细胞毒性。用福尔马林灭活的His-PLO进行疫苗接种可提供针对化脓隐秘杆菌攻击的保护作用，这表明PLO既是一种宿主保护性抗原，也是一种毒力决定因素。
相似文献
1
An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence.一种化脓隐秘杆菌（放线菌）突变体，其形成孔道的细胞溶素化脓素的产生存在缺陷，毒力降低。
Infect Immun. 1999 Apr;67(4):1723-8. doi: 10.1128/IAI.67.4.1723-1728.1999.
2
Molecular characterization of the pore-forming toxin, pyolysin, a major virulence determinant of Arcanobacterium pyogenes.化脓隐秘杆菌主要毒力决定因素——成孔毒素溶血素的分子特征
Vet Microbiol. 2001 Sep 28;82(3):261-74. doi: 10.1016/s0378-1135(01)00373-x.
3
The Arcanobacterium (Actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiol-activated cytolysin family.化脓隐秘杆菌（放线菌属）溶血素——化脓素，是硫醇激活溶血素家族的一个新成员。
J Bacteriol. 1997 Oct;179(19):6100-6. doi: 10.1128/jb.179.19.6100-6106.1997.
4
Immunization with genetic toxoids of the Arcanobacterium pyogenes cholesterol-dependent cytolysin, pyolysin, protects mice against infection.用化脓隐秘杆菌胆固醇依赖性细胞溶素（化脓菌素）的基因类毒素进行免疫接种可保护小鼠免受感染。
Infect Immun. 2003 May;71(5):2966-9. doi: 10.1128/IAI.71.5.2966-2969.2003.
5
Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin.用精氨酸取代 238 位天冬氨酸会损害溶葡萄球菌素的寡聚化活性和致炎特性。
Virulence. 2018;9(1):1112-1125. doi: 10.1080/21505594.2018.1491256.
6
The variant undecapeptide sequence of the Arcanobacterium pyogenes haemolysin, pyolysin, is required for full cytolytic activity.化脓隐秘杆菌溶血素（化脓菌素）的十一肽变异序列是完全细胞溶解活性所必需的。
Microbiology (Reading). 2002 Dec;148(Pt 12):3947-3954. doi: 10.1099/00221287-148-12-3947.
7
The gene encoding pyolysin, the pore-forming toxin of Arcanobacterium pyogenes, resides within a genomic islet flanked by essential genes.
FEMS Microbiol Lett. 2003 Aug 29;225(2):241-7. doi: 10.1016/S0378-1097(03)00527-5.
8
Transcriptional regulation of pyolysin production in the animal pathogen, Arcanobacterium pyogenes.
Vet Microbiol. 2008 Nov 25;132(1-2):96-104. doi: 10.1016/j.vetmic.2008.04.025. Epub 2008 Apr 24.
9
Differential endometrial cell sensitivity to a cholesterol-dependent cytolysin links Trueperella pyogenes to uterine disease in cattle.子宫内膜细胞对胆固醇依赖性细胞溶素的不同敏感性将化脓放线杆菌与牛的子宫疾病联系起来。
Biol Reprod. 2014 Mar 13;90(3):54. doi: 10.1095/biolreprod.113.115972. Print 2014 Mar.
10
Virulence markers associated with Trueperella pyogenes infections in livestock and companion animals.与家畜和伴侣动物化脓隐秘杆菌感染相关的毒力标志物
Lett Appl Microbiol. 2017 Aug;65(2):125-132. doi: 10.1111/lam.12757.
引用本文的文献
1
Virulence factors and therapeutic methods of : A review.《……的毒力因子与治疗方法：综述》
需注意，原文中冒号前缺少具体所指内容，翻译后的中文根据语境推测补充了“……”。
Virulence. 2025 Dec;16(1):2467161. doi: 10.1080/21505594.2025.2467161. Epub 2025 Feb 21.
2
Trueperella Pyogenes-Strain Diversity and Occurrence in Dairy Herds.化脓放线杆菌——奶牛群中的菌株多样性与发生情况
Pathogens. 2024 Jun 24;13(7):534. doi: 10.3390/pathogens13070534.
3
Revealing the Mechanism of NLRP3 Inflammatory Pathway Activation through K Efflux Induced by PLO via Signal Point Mutations.揭示 PLO 通过信号点突变诱导的 K+外流激活 NLRP3 炎症通路的机制。
Int J Mol Sci. 2024 Jun 18;25(12):6703. doi: 10.3390/ijms25126703.
4
Phenotypic Characteristics, Antimicrobial Susceptibility and Virulence Genotype Features of Associated with Endometritis of Dairy Cows.奶牛子宫内膜炎相关 的表型特征、药敏性和毒力基因型特征。
Int J Mol Sci. 2024 Apr 3;25(7):3974. doi: 10.3390/ijms25073974.
5
Immunoinformatics design of novel multi-epitope vaccine against Trueperella Pyogenes using collagen adhesion protein, fimbriae, and pyolysin.基于胶原蛋白黏附蛋白、菌毛和胞外蛋白酶设计抗化脓隐秘杆菌新型多表位疫苗的免疫信息学
Arch Microbiol. 2024 Feb 5;206(3):90. doi: 10.1007/s00203-023-03814-3.
6
Construction of live-attenuated Trueperella pyogenes by antibiotic treatment and sequential passage: methods for vaccine development.通过抗生素处理和连续传代构建减毒化脓隐秘杆菌：疫苗开发方法。
Arch Microbiol. 2023 Mar 27;205(4):147. doi: 10.1007/s00203-023-03481-4.
7
Comparative Genome Analysis of 19 Strains Originating from Different Animal Species Reveal a Genetically Diverse Open Pan-Genome.对来自不同动物物种的19株菌株进行的比较基因组分析揭示了一个遗传多样的开放泛基因组。
Antibiotics (Basel). 2022 Dec 24;12(1):24. doi: 10.3390/antibiotics12010024.
8
TatD DNases Contribute to Biofilm Formation and Virulence in .TatD脱氧核糖核酸酶有助于生物膜形成和在……中的毒力。 （原文不完整，此为根据现有内容的翻译）
Front Microbiol. 2021 Nov 15;12:758465. doi: 10.3389/fmicb.2021.758465. eCollection 2021.
9
Trueperella pyogenes pyolysin inhibits lipopolysaccharide-induced inflammatory response in endometrium stromal cells via autophagy- and ATF6-dependent mechanism.化脓隐秘杆菌溶血素通过自噬和ATF6依赖机制抑制脂多糖诱导的子宫内膜基质细胞炎症反应。
Braz J Microbiol. 2021 Jun;52(2):939-952. doi: 10.1007/s42770-021-00422-5. Epub 2021 Jan 16.
10
Differences in phenotypic and genetic characteristics of Trueperella pyogenes detected in slaughtered cattle and pigs with septicemia.从患有败血病的屠宰牛和猪中分离出的化脓隐秘杆菌的表型和遗传特征的差异。
J Vet Med Sci. 2020 May 20;82(5):626-631. doi: 10.1292/jvms.19-0370. Epub 2020 Mar 24.
本文引用的文献
1
Field trials with Corynebacterium, pyogenes alum-precipitated toxoid.
J Comp Pathol. 1950 Jul;60(3):225-9. doi: 10.1016/s0368-1742(50)80021-8.
2
3
Interaction of Yersinia enterocolitica with macrophages leads to macrophage cell death through apoptosis.小肠结肠炎耶尔森菌与巨噬细胞的相互作用会通过凋亡导致巨噬细胞死亡。
Infect Immun. 1997 Nov;65(11):4813-21. doi: 10.1128/iai.65.11.4813-4821.1997.
4
Electroporation-mediated transformation of Arcanobacterium (Actinomyces) pyogenes.电穿孔介导的化脓隐秘杆菌（放线菌属）转化
Plasmid. 1997;38(2):135-40. doi: 10.1006/plas.1997.1299.
5
The Arcanobacterium (Actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiol-activated cytolysin family.化脓隐秘杆菌（放线菌属）溶血素——化脓素，是硫醇激活溶血素家族的一个新成员。
J Bacteriol. 1997 Oct;179(19):6100-6. doi: 10.1128/jb.179.19.6100-6106.1997.
6
Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium phocae sp. nov., Arcanobacterium bernardiae comb. nov., and Arcanobacterium pyogenes comb. nov.基于16S rRNA基因序列的放线菌属系统发育分析：海豹隐秘杆菌新种、伯纳隐秘杆菌新组合及化脓隐秘杆菌新组合的描述
Int J Syst Bacteriol. 1997 Jan;47(1):46-53. doi: 10.1099/00207713-47-1-46.
7
Purification and further characterization of a haemolysin of Actinomyces pyogenes.
Zentralbl Veterinarmed B. 1996 May;43(3):179-88. doi: 10.1111/j.1439-0450.1996.tb00303.x.
8
Protection of experimentally infected pigs by suilysin, the thiol-activated haemolysin of Streptococcus suis.猪链球菌硫醇激活溶血素——溶菌酶对实验感染猪的保护作用
Vet Rec. 1996 Sep 7;139(10):225-8. doi: 10.1136/vr.139.10.225.
9
Apoptosis of mouse dendritic cells is triggered by listeriolysin, the major virulence determinant of Listeria monocytogenes.小鼠树突状细胞的凋亡是由李斯特菌溶血素触发的，李斯特菌溶血素是单核细胞增生李斯特菌的主要毒力决定因素。
Mol Microbiol. 1996 Apr;20(1):119-26. doi: 10.1111/j.1365-2958.1996.tb02494.x.
10
Pathogenesis of sepsis syndrome: possible relevance of pore-forming bacterial toxins.
Curr Top Microbiol Immunol. 1996;216:101-18. doi: 10.1007/978-3-642-80186-0_5.
Zotero 插件