Wente Nicole, Leimbach Stefanie, Woudstra Svenja, Krömker Volker
Department of Bioprocess Engineering and Microbiology, University of Applied Sciences and Arts Hannover, D-30453 Hannover, Germany.
Steinbeis Research Center Milk Science, D-32278 Kirchlengern, Germany.
Pathogens. 2024 Jun 24;13(7):534. doi: 10.3390/pathogens13070534.
is a mastitis-causing pathogen formerly known to cause severe clinical mastitis (CM), especially during the summer, leading to milk losses and low recovery rates. Unfortunately, its transmission behavior within herds is unclear. The diversity and occurrence of were monitored to gain an initial insight into the infection transmission behavior of in dairy herds and to lay a foundation for following targeted investigations. CM milk samples were collected from German herds, and one Swedish farm was sampled for isolates from subclinical mastitis. All in all, 151 isolates from 16 herds were isolated, identified by MALDI TOF analysis and typed with RAPD PCR. Of these, 17 isolates originated from subclinical mastitis cases. We found that mastitis occurred year-round, and clinical mastitis cases were caused by multiple strains (31 affected animals/28 strains). Instances of multiple cows being infected with the same strain were rare and typically only involved a small number of animals at a time. However, if several quarters of a cow were affected, it was likely the same strain. Unlike clinical infections, subclinical infections, in one investigated farm, harbored a dominant strain. Additionally, we found that infections tended to persist and stay within a herd for a minimum of 7 months in the same or different cows.
是一种曾被认为会引发严重临床型乳腺炎（CM）的致乳腺炎病原体，尤其是在夏季，会导致牛奶产量损失和低治愈率。不幸的是，其在牛群中的传播行为尚不清楚。对其多样性和出现情况进行监测，以便初步了解其在奶牛群中的感染传播行为，并为后续的针对性调查奠定基础。从德国牛群中采集临床型乳腺炎牛奶样本，并从一家瑞典农场采集亚临床型乳腺炎的分离株样本。总共从16个牛群中分离出151株 分离株，通过基质辅助激光解吸电离飞行时间质谱分析进行鉴定，并用随机扩增多态性DNA聚合酶链反应进行分型。其中，17株分离株源自亚临床型乳腺炎病例。我们发现 乳腺炎全年都会发生，临床型乳腺炎病例由多种菌株引起（31头患病动物/28种菌株）。同一头奶牛被相同 菌株感染的情况很少见，且通常一次只涉及少数动物。然而，如果一头奶牛的几个乳腺象限受到影响，则很可能是同一菌株。与临床感染不同，在一个被调查的农场中，亚临床 感染存在一种优势菌株。此外，我们发现 感染往往会持续存在于牛群中，在同一头或不同奶牛中至少持续7个月。
