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β4整合素相互作用蛋白p27（BBP/eIF6）是一种参与60S核糖体亚基组装的重要核基质蛋白。

The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly.

作者信息

Sanvito F, Piatti S, Villa A, Bossi M, Lucchini G, Marchisio P C, Biffo S

机构信息

DIBIT, Department of Biological and Technological Research, San Raffaele Scientific Institute, Milano, Italy.

出版信息

J Cell Biol. 1999 Mar 8;144(5):823-37. doi: 10.1083/jcb.144.5.823.

DOI:10.1083/jcb.144.5.823
PMID:10085284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2148184/
Abstract

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), an interactor of the cytoplasmic domain of integrin beta4 and, independently, as the putative translation initiation factor eIF6. To establish the in vivo function of p27(BBP/eIF6), its topographical distribution was investigated in mammalian cells and the effects of disrupting the corresponding gene was studied in the budding yeast, Saccharomyces cerevisiae. In epithelial cells containing beta4 integrin, p27(BBP/eIF6) is present in the cytoplasm and enriched at hemidesmosomes with a pattern similar to that of beta4 integrin. Surprisingly, in the absence and in the presence of the beta4 integrin subunit, p27(BBP/eIF6) is in the nucleolus and associated with the nuclear matrix. Deletion of the IIH S. cerevisiae gene, encoding the yeast p27(BBP/eIF6) homologue, is lethal, and depletion of the corresponding gene product is associated with a dramatic decrease of the level of free ribosomal 60S subunit. Furthermore, human p27(BBP/eIF6) can rescue the lethal effect of the iihDelta yeast mutation. The data obtained in vivo suggest an evolutionarily conserved function of p27(BBP/eIF6) in ribosome biogenesis or assembly rather than in translation. A further function related to the beta4 integrin subunit may have evolved specifically in higher eukaryotic cells.

摘要

p27(BBP/eIF6)是一种进化上保守的蛋白质，最初被鉴定为p27(BBP)，它是整合素β4胞质结构域的相互作用分子，同时独立地被鉴定为假定的翻译起始因子eIF6。为了确定p27(BBP/eIF6)在体内的功能，研究了其在哺乳动物细胞中的拓扑分布，并在芽殖酵母酿酒酵母中研究了破坏相应基因的影响。在含有β4整合素的上皮细胞中，p27(BBP/eIF6)存在于细胞质中，并在半桥粒处富集，其模式与β4整合素相似。令人惊讶的是，在不存在和存在β4整合素亚基的情况下，p27(BBP/eIF6)都位于核仁中并与核基质相关。编码酵母p27(BBP/eIF6)同源物的酿酒酵母IIH基因缺失是致死性的，相应基因产物的缺失与游离核糖体60S亚基水平的显著降低有关。此外，人p27(BBP/eIF6)可以挽救iihDelta酵母突变的致死效应。体内获得的数据表明p27(BBP/eIF6)在核糖体生物发生或组装中具有进化上保守的功能，而不是在翻译中。与β4整合素亚基相关的进一步功能可能是在高等真核细胞中特异性进化而来的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/756400406971/JCB9809068.f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/b8e86162151c/JCB9809068.f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/289b48638554/JCB9809068.f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/b76cff8964ab/JCB9809068.f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/7703823e6693/JCB9809068.f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/ae0d9911c278/JCB9809068.f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/a89a55ab8ff1/JCB9809068.f6a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/453bc2d08268/JCB9809068.f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/fa5cd382ad1a/JCB9809068.f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/8f9710beb57b/JCB9809068.f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/756400406971/JCB9809068.f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/b8e86162151c/JCB9809068.f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/289b48638554/JCB9809068.f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/b76cff8964ab/JCB9809068.f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/7703823e6693/JCB9809068.f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/ae0d9911c278/JCB9809068.f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/a89a55ab8ff1/JCB9809068.f6a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/453bc2d08268/JCB9809068.f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/fa5cd382ad1a/JCB9809068.f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/8f9710beb57b/JCB9809068.f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e47d/2148184/756400406971/JCB9809068.f10.jpg

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