荷兰人群亚组中的丙型GB病毒血症和包膜抗体

GB virus type C viremia and envelope antibodies among population subsets in The Netherlands.

作者信息

Jongerius J, Boland G, van der Poel C, Rasch M, Italiaander E, van der Reijden J, Friedman P, Cockerill J, van Leeuwen E, van Hattum J

机构信息

Blood Bank Midden-Nederland, Utrecht, The

出版信息

Vox Sang. 1999;76(2):81-4. doi: 10.1159/000031025.

DOI:10.1159/000031025

PMID:10085523

Abstract

BACKGROUND AND OBJECTIVES

Two new flaviviruses, hepatitis G virus and GB virus type C (GBV-C), are possible causative agents for non-A-E hepatitis. In this study we established the prevalence of GBV-C markers in various population subsets in The Netherlands by assays for GBV-C antibodies and GBV-C nucleic acid.

MATERIALS AND METHODS

We tested specimens from groups of patients with hepatitis of various causes, intravenous drug users (IVDUs), and blood donors for GBV-C RNA (LCx(R) GBV-C assay, Abbott Laboratories), and for antibodies to the GBV-C envelope E2 protein (GBV-C anti-E2) with an enzyme immunoassay (Abbott Laboratories). Patients and donors were represented in one group only.

RESULTS

GBV-C RNA and GBV-C anti-E2 prevalence were, respectively, 2/34 (6%) and 3/34 (9%) among patients with non-A-E hepatitis, 2/10 (20%) and 0/10 (0%) among hepatitis B virus patients, 10/40 (25%) and 19/40 (48%) among hepatitis C virus (HCV) patients, 1/8 (13%) and 0/8 (0%) among patients with autoimmune hepatitis (AIH), 24/102 (24%) and 72/102 (71%) among IVDUs, 1/34 (3%) and 2/34 (6%) among blood donors with indeterminate anti-HCV recombinant immunoblot assay reactivity, and 3/250 (1.2%) and 8/250 (3.2%) among first-time blood donors. The profile of simultaneous GBV-C RNA positivity plus GBV-C anti-E2 positivity was found in 2/40 (5%) HCV patients, 4/102 (4%) IVDUs, and 1/250 (0.4%) first time blood donors.

CONCLUSION

GBV-C infection appears not to be a major cause of non-A-E hepatitis and AIH, but is associated with parenteral risk. The prevalence of GBV-C viremia in first time blood donors is higher than that of HCV (1.2 vs. 0.04%), but GBV-C viremia in IVDUs is lower than HCV (24 vs. 59%). Most IVDUs have probably previously been exposed to GBV-C given the very high prevalence of GBV-C anti-E2 (71%). Most persons with GBV-C markers are GBV-C RNA-negative and anti-E2-confirmed positive, suggesting that GBV-C infection is transient.

摘要

背景与目的

两种新型黄病毒，即庚型肝炎病毒和丙型GB病毒（GBV-C），可能是非甲-戊型肝炎的致病原。在本研究中，我们通过检测GBV-C抗体和GBV-C核酸，确定了荷兰不同人群亚组中GBV-C标志物的流行情况。

材料与方法

我们使用LCx(R) GBV-C检测法（雅培实验室）检测了各类病因肝炎患者、静脉吸毒者（IVDUs）和献血者的标本中的GBV-C RNA，并使用酶免疫分析法（雅培实验室）检测了针对GBV-C包膜E2蛋白的抗体（GBV-C抗E2）。患者和献血者仅归为一组。

结果

在非甲-戊型肝炎患者中，GBV-C RNA和GBV-C抗E2的流行率分别为2/34（6%）和3/34（9%）；在乙型肝炎病毒患者中分别为2/10（20%）和0/10（0%）；在丙型肝炎病毒（HCV）患者中分别为10/40（25%）和19/40（48%）；在自身免疫性肝炎（AIH）患者中分别为1/8（13%）和0/8（0%）；在IVDUs中分别为24/102（24%）和72/102（71%）；在抗-HCV重组免疫印迹试验反应不确定的献血者中分别为1/34（3%）和2/34（6%）；在初次献血者中分别为3/250（1.2%）和8/250（3.2%）。在2/40（5%）的HCV患者、4/102（4%）的IVDUs和1/250（0.4%）的初次献血者中发现了GBV-C RNA阳性加GBV-C抗E2阳性的情况。

结论

GBV-C感染似乎不是非甲-戊型肝炎和AIH的主要病因，但与经肠外途径感染的风险相关。初次献血者中GBV-C病毒血症的流行率高于HCV（1.2%对0.04%），但IVDUs中GBV-C病毒血症低于HCV（24%对59%）。鉴于GBV-C抗E2的流行率非常高（71%），大多数IVDUs可能以前接触过GBV-C。大多数具有GBV-C标志物的人GBV-C RNA阴性且抗E2确认阳性，这表明GBV-C感染是短暂的。