灌注小鼠肝脏对半合成氚标记胰岛素的降解作用。
The degradation of semisynthetic tritiated insulin by perfused mouse livers.
作者信息
Halban P A, Karakash C, Davies J G, Offord R E
出版信息
Biochem J. 1976 Nov 15;160(2):409-12. doi: 10.1042/bj1600409.
Abstract
Semisynthetic [3H]insulin was stored under various conditions for up to 180 days and the stability of the insulin under these conditions was assessed. A sample that had been stored for 180 days was repurified and shown to be degraded at the same rate as native insulin by perfused mouse livers, even at low physiological concentrations. After perfusion, intact insulin could be separated from degradation products, and the radioactivity associated with the insulin fraction could be used to determine the percentage degradation. The initial rate of degradation of insulin was a linear function of concentration over the range 360pM-1.9nM.
摘要
半合成[3H]胰岛素在各种条件下储存长达180天，并评估了胰岛素在这些条件下的稳定性。一个储存了180天的样品被重新纯化，结果表明，即使在低生理浓度下，经灌注的小鼠肝脏对其降解速度与天然胰岛素相同。灌注后，完整的胰岛素可与降解产物分离，与胰岛素部分相关的放射性可用于确定降解百分比。在360pM-1.9nM范围内，胰岛素的初始降解速率是浓度的线性函数。
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