在半合成的[3H]甘氨酸A1胰岛素和[3H]苯丙氨酸B1胰岛素降解过程中，鉴定胰岛素蛋白酶释放的放射性胰岛素片段。
Identification of radioactive insulin fragments liberated by insulin proteinase during the degradation of semisynthetic [3H]GlyA1]insulin and [3H]PheB1]insulin.
作者信息
Davies J G, Muir A V, Rose K, Offord R E
机构信息
Département de Biochimie Médicale, Centre Médical Universitaire, Geneva, Switzerland.
出版信息
Biochem J. 1988 Jan 1;249(1):209-14. doi: 10.1042/bj2490209.
Abstract
(1) We [Muir, Offord & Davies (1986) Biochem. J. 237, 631-637 and Davies, Muir & Offord (1986) Biochem. J. 240, 609-612] have previously identified a major product in the degradation of insulin by insulin proteinase (the N-terminal fragment produced by cleavage between residues LeuA13 and TyrA14, SerB9 and HisB10) together with evidence for a minor cleavage site between HisB10 and LeuB11 or between LeuB11 and ValB12. (2) We now present evidence for minor sites of cleavage between TyrA14 and GlnA15, GluB13 and AlaB14 as well as HisB10 and LeuB11.
摘要
(1) 我们[缪尔、奥福德和戴维斯（1986年），《生物化学杂志》237卷，631 - 637页；以及戴维斯、缪尔和奥福德（1986年），《生物化学杂志》240卷，609 - 612页]先前已鉴定出胰岛素蛋白酶降解胰岛素的一种主要产物（由A链13位亮氨酸和14位酪氨酸、B链9位丝氨酸和10位组氨酸之间的切割产生的N端片段），同时有证据表明在B链10位组氨酸和11位亮氨酸之间或11位亮氨酸和12位缬氨酸之间存在一个次要切割位点。(2) 我们现在提供证据表明在A链14位酪氨酸和15位谷氨酰胺、B链13位谷氨酸和14位丙氨酸以及B链10位组氨酸和11位亮氨酸之间存在次要切割位点。
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Identification of radioactive insulin fragments liberated by insulin proteinase during the degradation of semisynthetic [3H]GlyA1]insulin and [3H]PheB1]insulin.在半合成的[3H]甘氨酸A1胰岛素和[3H]苯丙氨酸B1胰岛素降解过程中，鉴定胰岛素蛋白酶释放的放射性胰岛素片段。
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本文引用的文献
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Peptide intermediates in the cellular metabolism of insulin.胰岛素细胞代谢中的肽中间体。
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The fate of insulin in rat hepatocytes. Evidence for the release of an immunologically active fragment.胰岛素在大鼠肝细胞中的命运。释放具有免疫活性片段的证据。
Diabetes. 1984 Apr;33(4):355-61. doi: 10.2337/diab.33.4.355.
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Insulin binding and removal by livers of genetically obese rats.遗传性肥胖大鼠肝脏对胰岛素的结合与清除
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Characterization of a rat liver protease with specificity for insulin.一种对胰岛素具有特异性的大鼠肝脏蛋白酶的特性鉴定
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Purification of insulin-specific protease by affinity chromatography.通过亲和层析法纯化胰岛素特异性蛋白酶。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3698-702. doi: 10.1073/pnas.69.12.3698.
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The preparation of tritiated insulin specifically labelled by semisynthesis at glycine-A1.在甘氨酸 -A1 处通过半合成特异性标记的氚化胰岛素的制备。
Biochem J. 1985 Oct 15;231(2):389-92. doi: 10.1042/bj2310389.
8
Identification of an insulin fragment produced by an insulin degrading enzyme, neutral thiopeptidase.鉴定由胰岛素降解酶（中性硫肽酶）产生的一种胰岛素片段。
Mol Cell Endocrinol. 1987 Apr;50(3):171-5. doi: 10.1016/0303-7207(87)90014-1.
9
Identification of some cleavage sites of insulin by insulin proteinase.胰岛素蛋白酶对胰岛素某些裂解位点的鉴定。
Biochem J. 1986 Dec 1;240(2):609-12. doi: 10.1042/bj2400609.
10
The identification of a major product of the degradation of insulin by 'insulin proteinase' (EC 3.4.22.11).通过“胰岛素蛋白酶”（EC 3.4.22.11）鉴定胰岛素降解的主要产物。
Biochem J. 1986 Aug 1;237(3):631-7. doi: 10.1042/bj2370631.
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