Spagnoli L G, Palmieri G, Mauriello A, Orlandi A, Bancheri C, Pasetto N
Cattedra di Anatomia ed Istologia Patologica, Dipartimento di Chirurgia, II Universita' di Roma, Tor Vergata, Italy.
Atherosclerosis. 1990 May;82(1-2):27-36. doi: 10.1016/0021-9150(90)90140-e.
Female rabbits on a hypercholesterolemic atherogenic diet were treated with high doses of the synthetic progestogens norethisterone and medroxyprogesterone acetate in order to clarify the effect and possibly some of the mechanism of action of these hormones on diet-induced atherogenesis. We employed morphometric studies to determine the surface area of the rabbit aorta occupied by and the maximum thickness of lipid plaques. Autoradiography with tritiated thymidine was performed to demonstrate the effect of the progestogens on cell proliferation, which plays a key role in atherogenesis. Medroxyprogesterone acetate-treated and, above all, norethisterone-treated animals exhibit a more marked reduction of atherosclerosis than control rabbits fed the same diet. Our results suggest that both progestogens we used inhibit the development of atherosclerosis mainly by blocking the proliferation of smooth muscle cells in the tunica media and the cell population of the plaque.
为了阐明合成孕激素炔诺酮和醋酸甲羟孕酮对饮食诱导的动脉粥样硬化的作用及可能的一些作用机制，给食用高胆固醇致动脉粥样硬化饮食的雌性兔子使用了高剂量的上述两种合成孕激素。我们采用形态计量学研究来确定兔主动脉被脂质斑块占据的表面积和脂质斑块的最大厚度。进行了用氚标记胸腺嘧啶核苷的放射自显影，以证明孕激素对细胞增殖的影响，而细胞增殖在动脉粥样硬化中起关键作用。用醋酸甲羟孕酮处理的动物，尤其是用炔诺酮处理的动物，与喂食相同饮食的对照兔子相比，动脉粥样硬化的减轻更为明显。我们的结果表明，我们使用的两种孕激素主要通过阻断中膜平滑肌细胞和斑块细胞群的增殖来抑制动脉粥样硬化的发展。
