Sussman D, Greensides D, Reilly K, Wilson C
Department of Biology and Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):326-8. doi: 10.1107/S0907444998004922. Epub 1999 Jan 1.
A 35-nucleotide pseudoknot that binds vitamin B12 been isolated using systematic evolution of ligands by exponential enrichment (SELEX). Affinity chromatography was used to purify functional, properly folded molecules and the hanging-drop vapor-diffusion method was used to crystallize this aptamer. Two crystal forms have been obtained and the preliminary crystallographic characterization is reported here. Both crystal forms (space groups I222 or I212121 and C2221) diffract to 2.9 A and should prove sufficient for structure determination.
通过指数富集配体系统进化技术（SELEX）分离出了一种能结合维生素B12的35个核苷酸的假结。采用亲和色谱法纯化功能正常、折叠正确的分子，并使用悬滴气相扩散法使该适体结晶。已获得两种晶体形态，并在此报告了初步的晶体学表征。两种晶体形态（空间群I222或I212121以及C2221）的衍射分辨率均达到2.9埃，应该足以用于结构测定。
