Matsumoto O, Chen Y, Hasegawa S, Takenaka A, Ohta T, Koizumi M, Ohtsuka E
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symp Ser. 1993(29):171.
Crystallization of hammerhead ribozymes was tried by the hanging drop vapor diffusion method. The crystals grown up to 0.5 x 0.05 x 0.05 mm3 in size gave diffraction patterns with about 5 A resolution using synchrotron radiation. The crystal was determined to be trigonal with space group P3(1) or P3(2) and cell parameters a = b = 49.6 A and c = 53.3 A.
采用悬滴气相扩散法尝试使锤头状核酶结晶。通过同步辐射，生长至尺寸为0.5×0.05×0.05立方毫米的晶体给出了分辨率约为5埃的衍射图谱。该晶体被确定为三方晶系，空间群为P3(1)或P3(2)，晶胞参数a = b = 49.6埃，c = 53.3埃。
