Djinović Carugo K, Saraste M, Oka T
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69012 Heidelberg, Germany.
Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):667-8. doi: 10.1107/s0907444998010610.
Perchloric acid soluble protein purified from the cytosol fraction of rat liver has been crystallized in a form suitable for high-resolution X-ray diffraction studies. Octahedral crystals reaching 0.5 mm in cross-sectional diameter were produced by the hanging-drop method using polyethylene glycol (Mr = 8 kDa) as precipitant. These crystals diffract to 2.44 A on an in-house X-ray source and to 1.8 A using a bending-magnet beamline at ESRF Grenoble. The crystals belong to the cubic space group P213 with a = 89.90 A and two molecules per asymmetric unit, as indicated from a Vm value of 2.12 A3 Da-1 and self-rotation function computation. Screening for heavy-atom derivatives identified a platinum compound and xenon that bind to the protein.
从大鼠肝脏胞质溶胶部分纯化得到的高氯酸可溶性蛋白质已结晶成适合高分辨率X射线衍射研究的形式。采用悬滴法，以聚乙二醇（分子量 = 8 kDa）作为沉淀剂，制备出横截面直径达0.5毫米的八面体晶体。这些晶体在内部X射线源上的衍射分辨率为2.44 Å，在法国格勒诺布尔欧洲同步辐射装置（ESRF）的弯转磁铁光束线上的衍射分辨率为1.8 Å。根据2.12 ų Da⁻¹的Vm值和自旋转函数计算结果表明，这些晶体属于立方空间群P2₁3，晶胞参数a = 89.90 Å，每个不对称单元中有两个分子。对重原子衍生物的筛选鉴定出了与该蛋白质结合的铂化合物和氙。
