Nieuwenhuijs V B, van Duijvenbode-Beumer H, Verheem A, Visser M R, Verhoef J, Gooszen H G, Akkermans L M
Department of Surgery, University Hospital Utrecht, The Netherlands.
Eur J Clin Invest. 1999 Jan;29(1):33-40. doi: 10.1046/j.1365-2362.1999.00364.x.
Interdigestive small bowel motility has a regulatory function on the microflora of the upper small bowel. Here we investigate the effects of ABT-229 and octreotide on morphine-induced dysmotility, the accompanying bacterial overgrowth and bacterial translocation.
Rats were fitted with jejunal myoelectrodes and a subcutaneous cannula for continuous infusion of saline or morphine. Fasting motility was measured for 6 h on four occasions: one control measurement (day 0) and three measurements on consecutive days (days 1-3) while receiving saline alone (group A), morphine alone (group B), saline + ABT-229 (group C), morphine + ABT-229 (group D), saline + octreotide (group E) or morphine + octreotide (group F). Samples from the mesenteric lymph node complex (MLN), liver, spleen, duodenum and ileum were taken for quantitative microbial culturing on day 4.
Neither ABT-229 nor octreotide increased the number of propagated activity fronts during saline infusion. During morphine-induced dysmotility, ABT-229 induced more propagated activity fronts in group D (13.4, 9.8 and 8.8 per 6 h) than in group B (7.0, 4.5, 3.8 per 6 h) on days 1, 2 and 3 (P < 0.05 for all days) Octreotide did not induce more propagated activity fronts. Disruption of small bowel motility by morphine led to bacterial overgrowth in the duodenum. ABT-229 and octreotide did not reduce the bacterial growth levels. The total incidence of bacterial translocation was significantly higher in the morphine-treated animals than in the saline-treated animals. Neither ABT-229 nor octreotide reduced the bacterial translocation incidence. The number of propagated activity fronts on day 3 and duodenal bacterial growth correlated significantly in groups A, E and F.
ABT-229, but not octreotide, reduced morphine induced dysmotility. Small bowel bacterial overgrowth and bacterial translocation were not prevented. Fasting small bowel motility has a regulatory function on the intestinal microflora of the upper small bowel.
消化间期小肠动力对十二指肠上段的微生物群具有调节功能。在此，我们研究ABT - 229和奥曲肽对吗啡诱导的动力障碍、伴随的细菌过度生长及细菌移位的影响。
给大鼠安装空肠肌电电极及皮下插管，以便持续输注生理盐水或吗啡。在以下四种情况下测量禁食状态下的动力6小时：一次对照测量（第0天）以及连续三天（第1 - 3天）的三次测量，期间分别单独接受生理盐水（A组）、单独接受吗啡（B组）、生理盐水 + ABT - 229（C组）、吗啡 + ABT - 229（D组）、生理盐水 + 奥曲肽（E组）或吗啡 + 奥曲肽（F组）。在第4天采集肠系膜淋巴结复合体（MLN）、肝脏、脾脏、十二指肠和回肠的样本进行定量微生物培养。
在输注生理盐水期间，ABT - 229和奥曲肽均未增加传播性活动波峰的数量。在吗啡诱导的动力障碍期间，第1、2和3天，D组（每6小时分别为13.4、9.8和8.8个）中ABT - 229诱导的传播性活动波峰比B组（每6小时分别为7.0、4.5、3.8个）更多（所有天数P < 0.05）。奥曲肽未诱导出更多的传播性活动波峰。吗啡导致的小肠动力紊乱致使十二指肠细菌过度生长。ABT - 229和奥曲肽均未降低细菌生长水平。吗啡处理组动物的细菌移位总发生率显著高于生理盐水处理组动物。ABT - 229和奥曲肽均未降低细菌移位发生率。A组、E组和F组中，第3天的传播性活动波峰数量与十二指肠细菌生长显著相关。
ABT - 229而非奥曲肽减轻了吗啡诱导的动力障碍。小肠细菌过度生长和细菌移位未得到预防。禁食状态下的小肠动力对十二指肠上段的肠道微生物群具有调节功能。
