通过对拟南芥4号染色体上APETALA2基因座的一个400 kb连续片段进行测序和分析，获得拟南芥古老染色体复制的证据。
Evidence for an ancient chromosomal duplication in Arabidopsis thaliana by sequencing and analyzing a 400-kb contig at the APETALA2 locus on chromosome 4.
作者信息
Terryn N, Heijnen L, De Keyser A, Van Asseldonck M, De Clercq R, Verbakel H, Gielen J, Zabeau M, Villarroel R, Jesse T, Neyt P, Hogers R, Van Den Daele H, Ardiles W, Schueller C, Mayer K, Déhais P, Rombauts S, Van Montagu M, Rouzé P, Vos P
机构信息
Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Ghent, Belgium.
出版信息
FEBS Lett. 1999 Feb 26;445(2-3):237-45. doi: 10.1016/s0014-5793(99)00097-6.
As part of the European Scientists Sequencing Arabidopsis program, a contiguous region (396607 bp) located on chromosome 4 around the APETALA2 gene was sequenced. Analysis of the sequence and comparison to public databases predicts 103 genes in this area, which represents a gene density of one gene per 3.85 kb. Almost half of the genes show no significant homology to known database entries. In addition, the first 45 kb of the contig, which covers 11 genes, is similar to a region on chromosome 2, as far as coding sequences are concerned. This observation indicates that ancient duplications of large pieces of DNA have occurred in Arabidopsis.
作为欧洲科学家对拟南芥进行测序计划的一部分，对位于第4号染色体上APETALA2基因周围的一个连续区域（396607碱基对）进行了测序。对该序列的分析以及与公共数据库的比较预测，该区域有103个基因，基因密度为每3.85千碱基一个基因。几乎一半的基因与已知数据库条目没有显著同源性。此外，就编码序列而言，该重叠群的前45千碱基覆盖11个基因，与第2号染色体上的一个区域相似。这一观察结果表明，拟南芥中发生了大片段DNA的古老重复。
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