通过高丝氨酸激酶突变实现拟南芥对霜霉病的抗性
Downy mildew resistance in Arabidopsis by mutation of HOMOSERINE KINASE.
作者信息
van Damme Mireille, Zeilmaker Tieme, Elberse Joyce, Andel Annemiek, de Sain-van der Velden Monique, van den Ackerveken Guido
机构信息
Plant-Microbe Interactions, Department of Biology, Utrecht University, 3584 CH Utrecht, The Netherlands.
出版信息
Plant Cell. 2009 Jul;21(7):2179-89. doi: 10.1105/tpc.109.066811. Epub 2009 Jul 21.
Abstract
Plant disease resistance is commonly triggered by early pathogen recognition and activation of immunity. An alternative form of resistance is mediated by recessive downy mildew resistant 1 (dmr1) alleles in Arabidopsis thaliana. Map-based cloning revealed that DMR1 encodes homoserine kinase (HSK). Six independent dmr1 mutants each carry a different amino acid substitution in the HSK protein. Amino acid analysis revealed that dmr1 mutants contain high levels of homoserine that is undetectable in wild-type plants. Surprisingly, the level of amino acids downstream in the aspartate (Asp) pathway was not reduced in dmr1 mutants. Exogenous homoserine does not directly affect pathogen growth but induces resistance when infiltrated in Arabidopsis. We provide evidence that homoserine accumulation in the chloroplast triggers a novel form of downy mildew resistance that is independent of known immune responses.
摘要
植物抗病性通常由早期病原体识别和免疫激活引发。另一种抗性形式由拟南芥中的隐性霜霉病抗性1（dmr1）等位基因介导。基于图谱的克隆表明，DMR1编码高丝氨酸激酶（HSK）。六个独立的dmr1突变体在HSK蛋白中各自携带不同的氨基酸取代。氨基酸分析表明，dmr1突变体含有高水平的高丝氨酸，而野生型植物中则无法检测到。令人惊讶的是，天冬氨酸（Asp）途径下游的氨基酸水平在dmr1突变体中并未降低。外源高丝氨酸不会直接影响病原体生长，但在拟南芥中浸润时会诱导抗性。我们提供的证据表明，叶绿体中高丝氨酸的积累引发了一种新型的霜霉病抗性，该抗性独立于已知的免疫反应。
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本文引用的文献
1
Genome-scale proteomics reveals Arabidopsis thaliana gene models and proteome dynamics.全基因组蛋白质组学揭示拟南芥基因模型和蛋白质组动态变化。
Science. 2008 May 16;320(5878):938-41. doi: 10.1126/science.1157956. Epub 2008 Apr 24.
2
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.分选信号、N端修饰与叶绿体蛋白质组丰度
PLoS One. 2008 Apr 23;3(4):e1994. doi: 10.1371/journal.pone.0001994.
3
Arabidopsis DMR6 encodes a putative 2OG-Fe(II) oxygenase that is defense-associated but required for susceptibility to downy mildew.拟南芥DMR6编码一种假定的2-氧戊二酸-铁（II）加氧酶，该酶与防御相关，但对霜霉病的易感性是必需的。
Plant J. 2008 Jun;54(5):785-93. doi: 10.1111/j.1365-313X.2008.03427.x. Epub 2008 Jan 31.
4
The plant immune system.植物免疫系统。
Nature. 2006 Nov 16;444(7117):323-9. doi: 10.1038/nature05286.
5
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).黑杨（毛果杨，Populus trichocarpa (Torr. & Gray)）的基因组。
Science. 2006 Sep 15;313(5793):1596-604. doi: 10.1126/science.1128691.
6
Tête à tête inside a plant cell: establishing compatibility between plants and biotrophic fungi and oomycetes.植物细胞内的促膝长谈：建立植物与活体营养型真菌和卵菌之间的兼容性
New Phytol. 2006;171(4):699-718. doi: 10.1111/j.1469-8137.2006.01829.x.
7
Conserved requirement for a plant host cell protein in powdery mildew pathogenesis.白粉病发病机制中对植物宿主细胞蛋白的保守需求。
Nat Genet. 2006 Jun;38(6):716-20. doi: 10.1038/ng1806. Epub 2006 May 28.
8
The role of transamination in the synthesis of homoserine in peas.转氨基作用在豌豆中天冬氨酸同型半胱氨酸合成中的作用。
Plant Physiol. 1986 Sep;82(1):99-102. doi: 10.1104/pp.82.1.99.
9
Photosynthetic formation of the aspartate family of amino acids in isolated chloroplasts.离体叶绿体中氨基酸天冬氨酸族的光合形成
Plant Physiol. 1980 Jun;65(6):1166-72. doi: 10.1104/pp.65.6.1166.
10
Host-microbe interactions: shaping the evolution of the plant immune response.宿主-微生物相互作用：塑造植物免疫反应的进化
Cell. 2006 Feb 24;124(4):803-14. doi: 10.1016/j.cell.2006.02.008.
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