Fernández-Pérez F J, Méndez S, de la Fuente C, Cuquerella M, Gómez M T, Alunda J M
Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
J Vet Diagn Invest. 1999 Mar;11(2):170-3. doi: 10.1177/104063879901100211.
Specific serum antibody levels in Leishmania infantum-infected dogs treated with a combination of glucantime and allopurinol were estimated by indirect immunofluorescence and Western blotting. The sensitivity of Western blot was greater than that obtained with immunofluorescence titration. In general, both diagnostic methods concurred with the post-treatment clinical status of the animals. Clinical improvement of successfully treated dogs was related to lower immunofluorescence titers and simpler and/or less reactive immunodetection patterns in Western blotting. The recognition, by infected dogs, of certain low molecular weight antigens, particularly one of approximately 26 kDa, was restricted to pretreatment samples and a single animal in relapse thus apparently constituting an active infection marker.
采用间接免疫荧光法和蛋白质印迹法对接受葡糖胺和别嘌呤醇联合治疗的婴儿利什曼原虫感染犬的特异性血清抗体水平进行了评估。蛋白质印迹法的灵敏度高于免疫荧光滴定法。总体而言，两种诊断方法均与动物治疗后的临床状况相符。成功治疗的犬只临床症状改善与较低的免疫荧光滴度以及蛋白质印迹法中更简单和/或反应性更低的免疫检测模式相关。受感染犬只对某些低分子量抗原的识别，特别是约26 kDa的一种抗原，仅限于预处理样本以及一只复发动物，因此显然构成了活动性感染的标志物。
