Seyyedtabaei Seyyed Javad, Rostami Ali, Haghighi Ali, Mohebali Mehdi, Kazemi Bahram, Fallahi Shirzad, Spotin Adel
Dept. of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2017 Apr-Jun;12(2):206-214.
Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis.
This study was performed from 2010-2014 and participants were different parts of Iran. Serum samples were obtained from 43 patients with proven CL, 33 patients with proven VL, 39 patients with other parasitic diseases and 23 healthy individuals.
WB sensitivity for CL and VL was 100% and 91%, compared to IFA 4.6% and 87.8%, respectively. Sera from patients with CL and VL recognized numerous antigens with molecular weights ranging from 14 to 68 kDa and 12 to 94 kDa, respectively. The most sensitive antigens were 14 and 16 kDa for CL recognized by 100% of the sera from patients with proven CL and 12, 14 and 16 kDa for VL, recognized by 63.6%, 100% and 63.6% of the sera from patients with proven VL respectively. WB analysis is more sensitive than IFAT for the diagnosis of leishmaniasis particularly in cases of cutaneous leishmaniasis. The 12, 14 and 16 kDa can be valuable diagnostic molecules for serodiagnosis of leishmaniasis because at least two immunogenic molecules were simultaneously detected by all patient sera, as well as produced antibodies against these antigens have no cross-reactivity with other control groups.
WB could be useful for screening and serodiagnosis of CL and VL in epidemiologic studies in endemic areas.
内脏利什曼病（VL）和皮肤利什曼病（CL）是伊朗重要的公共卫生问题。我们旨在评估蛋白质印迹法（WB）与间接免疫荧光试验（IFAT）相比在利什曼病血清学诊断中的诊断潜力。
本研究于2010年至2014年进行，研究对象来自伊朗不同地区。从43例确诊为CL的患者、33例确诊为VL的患者、39例患有其他寄生虫病的患者和23名健康个体中获取血清样本。
WB对CL和VL的敏感性分别为100%和91%，而IFA对CL和VL的敏感性分别为4.6%和87.8%。CL和VL患者的血清分别识别出许多分子量范围为14至68 kDa和12至94 kDa的抗原。CL最敏感的抗原是14 kDa和16 kDa，确诊为CL的患者血清中有100%能识别；VL最敏感的抗原是12 kDa、14 kDa和16 kDa，确诊为VL的患者血清中分别有63.6%、100%和63.6%能识别。WB分析在利什曼病诊断中比IFAT更敏感，尤其是在皮肤利什曼病病例中。12 kDa、14 kDa和16 kDa可能是利什曼病血清学诊断中有价值的诊断分子，因为所有患者血清均能同时检测到至少两种免疫原性分子，且针对这些抗原产生的抗体与其他对照组无交叉反应。
WB可用于流行地区流行病学研究中CL和VL的筛查及血清学诊断。
