Pekary A E, Li H J, Chan S I, Hsu C J, Wagner T E
Biochemistry. 1975 Mar 25;14(6):1177-84. doi: 10.1021/bi00677a012.
The 220-MHz high-resolution proton magnetic resonance (PMR) spectrum of histone IV has been examined as a function of histone concentration, salt concentration, and pD. The hydrophobic C-terminal portion of the histone IV monomer appears to be largely PMR "invisible" indicating that this region of the polypeptide contains rigid secondary structure. Further loss of PMR resonance areas with increased histone IV concentration in neat D2O has been attributed to self-aggregation involving a monomer-dimer equilibrium. An equilibrium between the monomer and large aggregates, on the other hand, appears to dominate at NaCl concentrations above 0.01 M. pD studies reveal an abrupt increase in histone IV aggregation at pD smaller than 0.8 and precipitation of histone IV at pD values in the neighborhood of its isoelectric point, pD similar to 11.
已对组蛋白IV的220兆赫高分辨率质子磁共振（PMR）谱作为组蛋白浓度、盐浓度和pD的函数进行了研究。组蛋白IV单体的疏水C末端部分在很大程度上似乎是PMR“不可见的”，这表明多肽的该区域含有刚性二级结构。在纯D2O中，随着组蛋白IV浓度增加，PMR共振面积进一步减少归因于涉及单体-二聚体平衡的自聚集。另一方面，在NaCl浓度高于0.01 M时，单体与大聚集体之间的平衡似乎占主导。pD研究表明，在pD小于0.8时组蛋白IV聚集突然增加，并且在其等电点附近的pD值（pD约为11）时组蛋白IV沉淀。
