Beuling EE, Lens P, Ottengraf SP
Department of Chemical Engineering, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.
Biotechnol Bioeng. 1998 Nov 5;60(3):283-91. doi: 10.1002/(sici)1097-0290(19981105)60:3<283::aid-bit3>3.0.co;2-d.
The mobility of water in intact biofilms was measured with pulsed field gradient nuclear magnetic resonance (PFG-NMR) and used to characterise their diffusive properties. The results obtained with several well-defined systems, viz. pure water, agar, and agar containing inert particles or active bacteria were compared to glucose diffusion coefficients measured with micro-electrodes and those calculated utilising theoretical diffusion models. A good correspondence was observed indicating that PFG-NMR should also enable the measurement of diffusion coefficients in heterogeneous biological systems. Diffusion coefficients of several types of natural biofilms were measured as well and these results were related to the physical biofilm characteristics. The values had a high accuracy and reflected the properties of a sample of ca. 100 biofilms, while non-uniformity or non-geometrical shapes did not negatively influence the results. The monitored PFG-NMR signal contains supplementary information on e.g. cell fraction or spatial organisation but quantitative analysis was not yet possible. Copyright 1998 John Wiley & Sons, Inc.
采用脉冲场梯度核磁共振（PFG-NMR）技术测量完整生物膜中水的流动性，并用于表征其扩散特性。将几种定义明确的体系（即纯水、琼脂以及含有惰性颗粒或活性细菌的琼脂）所获得的结果，与用微电极测量的葡萄糖扩散系数以及利用理论扩散模型计算得到的扩散系数进行了比较。观察到良好的对应关系，这表明PFG-NMR也应能够测量非均相生物体系中的扩散系数。还测量了几种天然生物膜的扩散系数，并将这些结果与生物膜的物理特性相关联。这些值具有很高的准确性，反映了约100个生物膜样本的特性，而非均匀性或非几何形状并未对结果产生负面影响。监测到的PFG-NMR信号包含有关例如细胞分数或空间组织的补充信息，但尚未进行定量分析。版权所有1998约翰威立父子公司。
