Jolicoeur M, Williams RD, Chavarie C, Fortin JA, Archambault J
Ecole Polytechnique de Montreal, Department of Chemical Engineering, Biopro Research Centre, P.O. Box 6079 Centre-Ville Station, Montreal, Quebec, Canada, H3C 3A7.
Biotechnol Bioeng. 1999 Apr 20;63(2):224-32. doi: 10.1002/(sici)1097-0290(19990420)63:2<224::aid-bit11>3.0.co;2-x.
This work addresses the symbiotic culture of the arbuscular mycorrhizal (AM) fungus Glomus intraradices with Daucus carota hairy roots transformed by Agrobacterium rhizogenes, in two submerged culture systems: Petri dish and airlift bioreactor. AM fungi play an active role in plant nutrition and protection against plant pathogens. These fungi are obligate biotrophs as they depend on a host plant for their needs in carbohydrates. The effect of the mycorrhizal roots inoculum-to-medium volume ratio on the growth of both symbionts was studied. A critical inoculating condition was observed at approximately 0.6 g dry biomass (DW). L-1 medium, above which root growth was significantly reduced when using a low-salt minimal (M) liquid medium previously developed for hairy root-AM fungi co-culture. Below critical inoculum conditions the maximum specific root growth and specific G. intraradices spore production rates of 0.021 and 0.035 d-1, respectively, were observed for Petri dish cultures. Maximum spore production in the airlift bioreactor was ten times lower than that of Petri dish cultures and obtained with the lowest inoculum assessed (0.13 g DW. L-1 medium) with 1.82 x 10(5) +/- 4.05 x 10(4) (SEM) spores (g DW inoculum)-1 (L medium)-1 in 107 d. This work proposes a second-generation bioprocess for AM fungi propagule production in bioreactors. Copyright 1999 John Wiley & Sons, Inc.
本研究在两种深层培养系统（培养皿和空气提升式生物反应器）中，探讨了根内球囊霉（Glomus intraradices）这种丛枝菌根（AM）真菌与经发根农杆菌（Agrobacterium rhizogenes）转化的胡萝卜毛状根的共生培养。AM真菌在植物营养及抵御植物病原体方面发挥着积极作用。这些真菌是专性生物营养体，因为它们在碳水化合物需求上依赖宿主植物。研究了菌根根接种物与培养基体积比对两种共生体生长的影响。在使用先前为毛状根 - AM真菌共培养开发的低盐基本（M）液体培养基时，观察到约0.6 g干生物量（DW）·L⁻¹培养基为关键接种条件，高于此条件时根生长显著降低。在低于关键接种条件下，培养皿培养中观察到最大比根生长速率和根内球囊霉比孢子产生速率分别为0.021 d⁻¹和0.035 d⁻¹。空气提升式生物反应器中的最大孢子产量比培养皿培养低10倍，且是在评估的最低接种量（0.13 g DW·L⁻¹培养基）下获得的，在107天内为1.82×10⁵ ± 4.05×10⁴（SEM）孢子·（g DW接种物）⁻¹·（L培养基）⁻¹。本研究提出了一种用于在生物反应器中生产AM真菌繁殖体的第二代生物工艺。版权所有1999约翰威立父子公司。
