Drozdz M, Kucharz E, Ludyga K, Molska-Drozda T
Eur J Toxicol Environ Hyg. 1976 Sep-Oct;9(5):287-93.
Forty male guinea pigs were exposed to nitrogen dioxide in a concentration of 2 mg/m3, 8 hours daily for a period of 180 days. Forty male animals were used as a control group. The following changes were found in intoxicated animals: the decrease of total protein and seromucoid concentration in blood serum and the decrease of total protein, perchloric acid-soluble proteins, protein-bound hexosamines and sialic acids content, in liver tissue. Electrophoretic examination of the serum proteins showed the increase of alpha 1- and beta 2-globulins and the decrease of albumin concentration. Changes in the level of glycoproteins fractions and protein-bound carbohydrates in blood serum were described also. Estimation of enzymes activity showed the decrease of alanine and aspartate transaminase activity in blood serum caused by the strong decrease of the cytoplasmic fraction of these enzymes. However the simultaneous increase of the mitochondrial fraction of transaminases activity was observed. The decrease of the activity of choline esterase was found also. Similar changes of enzymes activity were found in liver tissue. Histopathological studies were done for the further clearing the influenze of nitrogen dioxide on serum and liver proteins concentration and enzymes activity. It was found that after long-term exposure to nitrogen dioxide the destruction processes may be observed in the liver. The possible mechanism of the nitrogen dioxide-induced damage of protein metabolism is discussed.
40只雄性豚鼠每天8小时暴露于浓度为2毫克/立方米的二氧化氮环境中，持续180天。40只雄性动物作为对照组。在中毒动物中发现了以下变化：血清中总蛋白和血清类粘蛋白浓度降低，肝组织中总蛋白、高氯酸可溶性蛋白、蛋白结合己糖胺和唾液酸含量降低。血清蛋白的电泳检查显示α1和β2球蛋白增加，白蛋白浓度降低。还描述了血清中糖蛋白组分和蛋白结合碳水化合物水平的变化。酶活性评估显示，由于这些酶的细胞质部分大幅减少，血清中丙氨酸和天冬氨酸转氨酶活性降低。然而，同时观察到转氨酶线粒体部分活性增加。还发现胆碱酯酶活性降低。在肝组织中也发现了类似的酶活性变化。进行了组织病理学研究，以进一步明确二氧化氮对血清和肝蛋白浓度及酶活性的影响。发现长期暴露于二氧化氮后，肝脏中可能会观察到破坏过程。讨论了二氧化氮诱导蛋白质代谢损伤的可能机制。
