Tomaszewski J, Zwierz K, Mnich Z, Jezyna C
Acta Biol Hung. 1985;36(1):15-23.
Male guinea-pigs weighing 400-600 g, 8 months old, were given metribuzin directly into the gastric lumen over a period of 30 days (20 animals) or 90 days (20 animals) 6 times a week. In the liver of the poisoned animals, the glycogen level and the AspAT and AlAT activities, while in the serum the total protein and the fractions albumin, alpha 1-globulin and gamma-globulin significantly decreased; serum glucose and the serum fractions alpha 2-globulin and beta-globulin, each showed an increase. The glycogen level in the liver, total protein, glucose as well as the alpha 1 and alpha 2 globulin fractions in the serum showed not appreciable difference between 30 and 90 days of intoxication. After 90 days of metribuzin treatment AspAT and AlAT dropped in the liver and rose in the serum, in comparison to the 30-day values. As to the parameters of glycoprotein metabolism, the intoxicated animals showed a significant decrease and increase in concentration of hexosamines and sialic acids in the liver and serum, respectively. Metribuzin intoxication also cause a significant decrease in activity of glucosamine phosphate isomerase and significant increase in activity of glycosidases in the liver. The results suggest that metribuzin disturbs the metabolism of carbohydrates, proteins and glycoproteins in the guinea-pig liver.
体重400 - 600克、8月龄的雄性豚鼠，每周6次，连续30天（20只动物）或90天（20只动物）经胃腔直接给予嗪草酮。中毒动物肝脏中的糖原水平、天冬氨酸氨基转移酶（AspAT）和丙氨酸氨基转移酶（AlAT）活性，以及血清中的总蛋白、白蛋白、α1球蛋白和γ球蛋白组分均显著降低；血清葡萄糖以及血清α2球蛋白和β球蛋白组分均升高。肝脏中的糖原水平、总蛋白、葡萄糖以及血清中的α1和α2球蛋白组分在中毒30天和90天之间无明显差异。与30天的值相比，嗪草酮治疗90天后，肝脏中的AspAT和AlAT下降，血清中则升高。关于糖蛋白代谢参数，中毒动物肝脏和血清中的己糖胺和唾液酸浓度分别显著降低和升高。嗪草酮中毒还导致肝脏中磷酸葡糖胺异构酶活性显著降低，糖苷酶活性显著升高。结果表明，嗪草酮扰乱了豚鼠肝脏中碳水化合物、蛋白质和糖蛋白的代谢。
