Salem L, Walter N, Malone R
Program in Genetics, University of Iowa, Iowa City, Iowa 52242, USA.
Genetics. 1999 Apr;151(4):1261-72. doi: 10.1093/genetics/151.4.1261.
REC104 is a gene required for the initiation of meiotic recombination in Saccharomyces cerevisiae. To better understand the role of REC104 in meiosis, we used an in vitro mutagenesis technique to create a set of temperature-conditional mutations in REC104 and used one ts allele (rec104-8) in a screen for high-copy suppressors. An increased dosage of the early exchange gene REC102 was found to suppress the conditional recombinational reduction in rec104-8 as well as in several other conditional rec104 alleles. However, no suppression was observed for a null allele of REC104, indicating that the suppression by REC102 is not "bypass" suppression. Overexpression of the early meiotic genes REC114, RAD50, HOP1, and RED1 fails to suppress any of the rec104 conditional alleles, indicating that the suppression might be specific to REC102.
REC104是酿酒酵母减数分裂重组起始所需的基因。为了更好地理解REC104在减数分裂中的作用，我们使用体外诱变技术在REC104中创建了一组温度条件突变，并在筛选高拷贝抑制子时使用了一个温度敏感等位基因（rec104-8）。发现早期交换基因REC102剂量增加可抑制rec104-8以及其他几个条件性rec104等位基因中的条件性重组减少。然而，对于REC104的无效等位基因未观察到抑制作用，这表明REC102的抑制不是“旁路”抑制。早期减数分裂基因REC114、RAD50、HOP1和RED1的过表达未能抑制任何rec104条件等位基因，这表明这种抑制可能是REC102特有的。
