Nau J J, Summers K R, Galbraith A M, Bullard S A, Malone R E
Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA.
Curr Genet. 1997 Jan;31(1):7-14. doi: 10.1007/s002940050170.
The REC104 gene of Saccharomyces cerevisiae is required to initiate recombination in meiosis. Mutations in REC104 eliminate meiotic recombination and lead to the production of inviable spores. To determine if analogous genes exist in other yeasts, clones that hybridized to a REC104 probe were isolated from the yeasts S. paradoxus and S. pastorianus. When transformed into a rec104 strain, the REC104 analogs from these two yeasts restored spore viability and meiotic recombination to the same level as a REC104 gene cloned from S. cerevisiae. Compared to S. cerevisiae, the S. paradoxus gene codes for 79% identical amino acids and has 86% nucleic-acid identity in the promoter region and 84% in the coding region. The S. pastorianus gene codes for 63% identical amino acids and has 59% and 71% identity in the promoter and the coding regions, respectively.
酿酒酵母的REC104基因是减数分裂中启动重组所必需的。REC104的突变会消除减数分裂重组，并导致产生不可育的孢子。为了确定其他酵母中是否存在类似基因，从奇异酵母和巴斯德酵母中分离出了与REC104探针杂交的克隆。当将这两种酵母的REC104类似物转化到rec104菌株中时，它们恢复了孢子活力和减数分裂重组，达到了从酿酒酵母中克隆的REC104基因的相同水平。与酿酒酵母相比，奇异酵母基因编码79%相同的氨基酸，启动子区域有86%的核酸同一性，编码区域有84%的核酸同一性。巴斯德酵母基因编码63%相同的氨基酸，启动子区域和编码区域分别有59%和71%的同一性。
