Malone R E, Bullard S, Hermiston M, Rieger R, Cool M, Galbraith A
Department of Biology, University of Iowa, Iowa City 52242.
Genetics. 1991 May;128(1):79-88. doi: 10.1093/genetics/128.1.79.
Using a selection based upon the ability of early Rec- mutations (e.g., rad50) to rescue the meiotic lethality of a rad52 spo13 strain, we have isolated 177 mutants. Analysis of 56 of these has generated alleles of the known Rec genes SPO11, ME14 and MER1, as well as defining five new genes: REC102, REC104, REC107, REC113 and REC114. Mutations in all of the new genes appear to specifically affect meiosis; they do not have any detectable mitotic phenotype. Mutations in REC102, REC104 and REC107 reduce meiotic recombination several hundred fold. No alleles of RED1 or HOP1 were isolated, consistent with the proposal that these genes may be primarily involved with chromosome pairing and not exchange.
利用基于早期Rec突变（如rad50）拯救rad52 spo13菌株减数分裂致死性的能力进行的筛选，我们分离出了177个突变体。对其中56个的分析产生了已知Rec基因SPO11、ME14和MER1的等位基因，同时还确定了五个新基因：REC102、REC104、REC107、REC113和REC114。所有新基因中的突变似乎都特异性地影响减数分裂；它们没有任何可检测到的有丝分裂表型。REC102、REC104和REC107中的突变使减数分裂重组减少了数百倍。未分离到RED1或HOP1的等位基因，这与这些基因可能主要参与染色体配对而非交换的提议一致。
