Pulatova M K, Sharygin V L, Todorov I N
Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygins str. 4, 117977, Semenov, Russia.
Biochim Biophys Acta. 1999 Mar 30;1453(3):321-9. doi: 10.1016/s0925-4439(99)00002-2.
Cellular requirements for deoxyribonucleotide (dNTP) pools during DNA synthesis are related to ensuring of the accuracy of DNA copying during replication and repair. This paper covers some problems on the reactions of dNTP synthesis system in organs of animals against the treatment with DNA-damaging agents. Ribonucleoside diphosphate reductase (NDPR) is the key enzyme for the synthesis of dNTP, since it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. The results obtained show that the rapid and transient increase in NDPR activity in animal organs occurs as cellular response against the treatment with DNA-damaging agents (SOS-type activation). We have also found the intensive radioprotector-stimulated activation of deoxyribonucleotide synthesis as well as DNA and protein synthesis in mice organs within 3 days after the administration of two radioprotectors, indralin and indometaphen, that provide the high animal survival. Our studies suggest that these effects are the most important steps in the protective mechanism of the radioprotectors and are responsible for the high animal survival.
DNA合成过程中对脱氧核糖核苷酸（dNTP）池的细胞需求与确保复制和修复过程中DNA复制的准确性有关。本文涵盖了动物器官中dNTP合成系统对DNA损伤剂处理的反应的一些问题。核糖核苷二磷酸还原酶（NDPR）是dNTP合成的关键酶，因为它催化核糖核苷酸向脱氧核糖核苷酸的还原转化。所得结果表明，动物器官中NDPR活性的快速和短暂增加是细胞对DNA损伤剂处理的反应（SOS型激活）。我们还发现，在给予两种能提高动物存活率的辐射防护剂吲哚拉林和吲哚美辛后3天内，小鼠器官中脱氧核糖核苷酸合成以及DNA和蛋白质合成受到辐射防护剂的强烈刺激而激活。我们的研究表明，这些效应是辐射防护剂保护机制中最重要的步骤，并导致动物的高存活率。
