Hengerer A, Decker J, Prohaska E, Hauck S, Kösslinger C, Wolf H
University of Regensburg, Institute for Medical Microbiology and Hygiene, Germany.
Biosens Bioelectron. 1999 Feb;14(2):139-44. doi: 10.1016/s0956-5663(98)00111-0.
An immunosensing system based on a quartz crystal microbalance (QCM) is presented for the selection of both antigen specific recombinant antibodies and antigen specific human pancreatic secretory trypsin inhibitor (hPSTI) mutants isolated from large phage libraries. The QCM was integrated into a flow injection analysis system for the straightforward analysis of large sample numbers. Measurements were performed using a biotinylated antigen immobilized by streptavidin onto the gold surface of the quartz crystal and phages displaying recombinant antibodies or hPSTI mutants. The results obtained by the QCM were in accordance to those of a well established enzyme linked immunosorbent assay (ELISA). Therefore, the QCM is well suited for the detection of single high affinity clones isolated from large phage display libraries.
本文介绍了一种基于石英晶体微天平（QCM）的免疫传感系统，用于从大型噬菌体文库中筛选抗原特异性重组抗体和抗原特异性人胰腺分泌型胰蛋白酶抑制剂（hPSTI）突变体。QCM被集成到流动注射分析系统中，以便直接分析大量样品。测量是使用通过链霉亲和素固定在石英晶体金表面的生物素化抗原来进行的，噬菌体展示重组抗体或hPSTI突变体。QCM获得的结果与成熟的酶联免疫吸附测定（ELISA）结果一致。因此，QCM非常适合检测从大型噬菌体展示文库中分离出的单个高亲和力克隆。
