Park C S, Kim J
Department of Pathology, Chonnam National University School of Medicine, Kwangju, Korea.
J Korean Med Sci. 1999 Feb;14(1):15-20. doi: 10.3346/jkms.1999.14.1.15.
Various in situ hybridization (ISH) methods have been used to identify Helicobacter pylori, a causative organism responsible for chronic gastritis and peptic ulcer disease, but they were hard to perform and time consuming. To detect H. pylori in a rapid and easily reproducible way, we developed synthetic biotinylated oligonucleotide probes which complement rRNA of H. pylori. Formalin-fixed and paraffin-embedded tissues from 50 gastric biopsy specimens were examined. Using a serologic test and histochemical stain (Warthin-Starry silver stain and/or Giemsa stain) as a standard, 40 of them were confirmed to be H. pylori-positive. Our ISH was quickly carried out within one hr and results were compared with those obtained from immunohistochemical stain. The ISH produced a positive reaction in 38 of 40 cases (95%). All H. pylori-negative cases failed to demonstrate a positive signal. The ISH has a sensitivity comparable to those of conventional histochemical and immunohistochemical stain, and has high specificity. In conclusion, ISH with a biotinylated oligonucleotide probe provides a useful diagnostic method for detecting H. pylori effectively in routinely processed tissue sections.
多种原位杂交（ISH）方法已被用于鉴定幽门螺杆菌，这是一种导致慢性胃炎和消化性溃疡病的病原体，但这些方法操作困难且耗时。为了以快速且易于重复的方式检测幽门螺杆菌，我们开发了与幽门螺杆菌rRNA互补的合成生物素化寡核苷酸探针。对50例胃活检标本的福尔马林固定石蜡包埋组织进行了检查。以血清学检测和组织化学染色（沃辛-斯塔里银染色和/或吉姆萨染色）作为标准，其中40例被确认为幽门螺杆菌阳性。我们的ISH在1小时内快速完成，并将结果与免疫组织化学染色结果进行比较。ISH在40例中的38例（95%）产生了阳性反应。所有幽门螺杆菌阴性病例均未显示阳性信号。ISH的敏感性与传统组织化学和免疫组织化学染色相当，且具有高特异性。总之，使用生物素化寡核苷酸探针的ISH为在常规处理的组织切片中有效检测幽门螺杆菌提供了一种有用的诊断方法。
