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兔乳腺酪蛋白的免疫化学特性分析

Immunochemical characterization of casein from rabbit mammary gland.

作者信息

Al-Sarraj K, White D A, Mayer R J

出版信息

Biochem J. 1978 Sep 1;173(3):877-83. doi: 10.1042/bj1730877.

DOI:10.1042/bj1730877
PMID:101209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185854/
Abstract
  1. Excellent precipitating antibodies to rabbit recombined casein polypeptides were obtained in a sheep after 8 weeks of immunization with rabbit recombined polypeptides coupled to Sepharose-albumin. 2. The antiserum was assessed for specificity by several immunochemical techniques and was monospecific when tested against acid-precipitated casein, recombined casein and extracts of lactating rabbit mammary tissue. 3. A specific anti-casein immunoglobulin fraction was prepared by immunoadsorption of the antiserum by using Sepharose-recombined casein as immunoadsorbent. 4. The specific anti-casein immunoglobulin was used to prepare a Sepharose-anti-casein immunoadsorbent for the isolation of casein from extracts of rabbit mammary tissue.
摘要
  1. 用与琼脂糖 - 白蛋白偶联的兔重组多肽免疫绵羊8周后，获得了针对兔重组酪蛋白多肽的优良沉淀抗体。2. 通过几种免疫化学技术评估抗血清的特异性，当针对酸沉淀酪蛋白、重组酪蛋白和泌乳兔乳腺组织提取物进行测试时，该抗血清具有单特异性。3. 通过使用琼脂糖 - 重组酪蛋白作为免疫吸附剂对抗血清进行免疫吸附，制备了特异性抗酪蛋白免疫球蛋白组分。4. 特异性抗酪蛋白免疫球蛋白用于制备琼脂糖 - 抗酪蛋白免疫吸附剂，以从兔乳腺组织提取物中分离酪蛋白。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a2/1185854/8ad45ccbde68/biochemj00481-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a2/1185854/2aaff71483d0/biochemj00481-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a2/1185854/8ad45ccbde68/biochemj00481-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a2/1185854/2aaff71483d0/biochemj00481-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47a2/1185854/8ad45ccbde68/biochemj00481-0184-a.jpg

相似文献

1
Immunochemical characterization of casein from rabbit mammary gland.兔乳腺酪蛋白的免疫化学特性分析
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2
Regulation of casein messenger RNA during the development of the rat mammary gland.大鼠乳腺发育过程中酪蛋白信使核糖核酸的调控
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Int J Biochem. 1978;9(4):269-77. doi: 10.1016/0020-711x(78)90009-5.

引用本文的文献

1
Glycoprotein synthesis in explants of developing rabbit mammary gland in culture.培养的发育中兔乳腺外植体中的糖蛋白合成
Biochem J. 1981 Sep 15;198(3):683-90. doi: 10.1042/bj1980683.
2
Protein degradation during terminal cytodifferentiation. Studies on mammary gland in organ culture.终末细胞分化过程中的蛋白质降解。器官培养中乳腺的研究。
Biochem J. 1980 Oct 15;192(1):311-20. doi: 10.1042/bj1920311.
3
Extensive destruction of newly synthesized casein in mammary explants in organ culture.器官培养中乳腺外植体中新合成酪蛋白的广泛破坏。

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A comparative immunologic and electrophoretic analysis of rat and mouse caseins.大鼠和小鼠酪蛋白的比较免疫学与电泳分析
Comp Biochem Physiol. 1970 Dec 1;37(3):421-7. doi: 10.1016/0010-406x(70)90570-0.
3
The content of casein in lactating mammary glands of the rat. Phosphoprotein assay on rennin-CA ++ precipitated proteins.大鼠乳腺中酪蛋白的含量。对凝乳酶-Ca++沉淀蛋白的磷蛋白分析。
Biochem J. 1982 Jan 15;202(1):133-8. doi: 10.1042/bj2020133.
4
Chemical and immunochemical characterization of caseins and the major whey proteins of rabbit milk.兔乳中酪蛋白和主要乳清蛋白的化学及免疫化学特性
Biochem J. 1982 Jan 1;201(1):71-9. doi: 10.1042/bj2010071.
5
Casein turnover in rabbit mammary explants in organ culture.器官培养中兔乳腺外植体的酪蛋白周转率
Biochem J. 1979 Sep 15;182(3):837-45. doi: 10.1042/bj1820837.
6
The effect of tunicamycin on the glycosylation of lactating-rabbit mammary glycoproteins.衣霉素对泌乳兔乳腺糖蛋白糖基化的影响。
Biochem J. 1979 Jun 15;180(3):481-9. doi: 10.1042/bj1800481.
7
Immunochemical characterization of monoamine oxidase from human liver, placenta, platelets and brain cortex.人肝脏、胎盘、血小板和大脑皮质中单胺氧化酶的免疫化学特性
Biochem J. 1979 Jul 1;181(1):15-20. doi: 10.1042/bj1810015.
Acta Endocrinol (Copenh). 1972 Feb;69(2):413-6. doi: 10.1530/acta.0.0690413.
4
Phospholipase A 1 activity of guinea pig pancreas.豚鼠胰腺的磷脂酶A1活性
Biochim Biophys Acta. 1971 Jul 21;242(1):99-107. doi: 10.1016/0005-2744(71)90091-x.
5
A direct radioimmunoassay for mouse casein.一种针对小鼠酪蛋白的直接放射免疫测定法。
Comp Biochem Physiol A Comp Physiol. 1974 Sep 1;49(1A):127-35. doi: 10.1016/0300-9629(74)90548-9.
6
Purification of the mRNAs for Ewe alphaS-casein and beta-casein by immunoprecipitation of polysomes.通过多核糖体免疫沉淀法纯化母羊αS-酪蛋白和β-酪蛋白的mRNA。
Eur J Biochem. 1976 Mar 16;63(1):9-14. doi: 10.1111/j.1432-1033.1976.tb10200.x.
7
An indirect radioimmunoassay for mouse casein using 125I-labeled antigen.一种使用¹²⁵I标记抗原的小鼠酪蛋白间接放射免疫测定法。
J Immunol Methods. 1975 Dec;9(2):123-34. doi: 10.1016/0022-1759(75)90102-7.
8
Regulation of enzyme turnover during tissue differention. Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture.组织分化过程中酶周转的调节。关于激素对器官培养的兔乳腺中脂肪酸合成酶周转影响的研究。
Biochem J. 1975 May;148(2):309-20. doi: 10.1042/bj1480309.
9
Regulation of casein synthesis in the rabbit mammary gland. Titration of mRNA activity for casein under prolactin and progesterone treatments.兔乳腺中酪蛋白合成的调节。催乳素和孕酮处理下酪蛋白mRNA活性的滴定。
Mol Cell Endocrinol. 1975 Jul;3(1):37-55. doi: 10.1016/0303-7207(75)90030-1.
10
Regulation of casein messenger RNA during the development of the rat mammary gland.大鼠乳腺发育过程中酪蛋白信使核糖核酸的调控
Biochemistry. 1975 Jul;14(13):2895-903. doi: 10.1021/bi00684a016.