Moger W H
Can J Physiol Pharmacol. 1975 Oct;53(5):839-44. doi: 10.1139/y75-115.
The ability of testosterone, androsterone, 5alpha-androstane-3alpha,17beta-diol, and 5alpha-androstane-3beta,17beta-diol to prevent the castration-induced rise in serum gonadotropin levels was investigated in immature male rats. Rats castrated at 30 days of age were treated once per day by subcutaneous injection of 12.5-100 mug of the steroid per 100 g body weight per day for 3 days, beginning on the day of castration. The animals were sacrificed 24 h after the last injection. Testosterone propionate, androsterone propionate, and 5alpha-androstane-3alpha,17beta-diol dipropionate were also tested at the approximate molar equivalent of 100 mug of the free alcohol form per 100 g body weight per day. Testosterone propionate and 5alpha-androstane-3alpha,17beta-diol were the only compounds tested that prevented the castration induced rise in luteinizing hormone (LH) concentrations. Testosterone propionate also inhibited the rise in follicle stimulating hormone (FSH) concentrations whereas 5alpha-androstane-3alpha,17beta-diol inhibited the rise in FSH in one but not in another experiment. These were the only compounds tested that affected serum FSH concentrations. The lower doses of testosterone tested significantly increased serum LH, but not FSH concentrations compared to castrate control animals. The highest dose tested partially inhibited the rise in serum LH concentrations. Both androsterone and androsterone propionate maintained ventral prostate weights. Although neither compound prevented the castration induced rise in serum LH, two groups receiving androsterone had serum LH concentrations significantly lower than the castrate control group. 5alpha-Androstane-3beta,17beta-diol and 5alpha-androstane-3alpha,17beta-diol dipropionate failed to maintain ventral prostate weights or prevent the rise in serum gonadotropin levels. These results indicate that 5alpha-androstane-3alpha,17beta-diol is capable of preventing the castration induced rise in serum LH concentrations in the immature male rat and thus may participate in the regulation of LH secretion in these animals.
在未成熟雄性大鼠中研究了睾酮、雄酮、5α-雄甾烷-3α,17β-二醇和5α-雄甾烷-3β,17β-二醇预防去势诱导的血清促性腺激素水平升高的能力。30日龄去势的大鼠从去势当天开始，每天皮下注射12.5 - 100μg/100g体重的类固醇，连续3天，每天注射一次。在最后一次注射后24小时处死动物。丙酸睾酮、丙酸雄酮和5α-雄甾烷-3α,17β-二醇二丙酸酯也以每天100μg游离醇形式每100g体重的近似摩尔当量进行了测试。丙酸睾酮和5α-雄甾烷-3α,17β-二醇是仅有的两种被测试能预防去势诱导的黄体生成素（LH）浓度升高的化合物。丙酸睾酮也抑制促卵泡激素（FSH）浓度的升高，而5α-雄甾烷-3α,17β-二醇在一个实验中抑制了FSH的升高，但在另一个实验中未抑制。这些是仅有的被测试影响血清FSH浓度的化合物。与去势对照动物相比，较低剂量的睾酮显著增加血清LH，但不增加FSH浓度。测试的最高剂量部分抑制了血清LH浓度的升高。雄酮和丙酸雄酮都维持了腹侧前列腺重量。尽管这两种化合物都没有预防去势诱导的血清LH升高，但接受雄酮的两组血清LH浓度显著低于去势对照组。5α-雄甾烷-3β,17β-二醇和5α-雄甾烷-3α,17β-二醇二丙酸酯未能维持腹侧前列腺重量或预防血清促性腺激素水平的升高。这些结果表明，5α-雄甾烷-3α,17β-二醇能够预防未成熟雄性大鼠去势诱导的血清LH浓度升高，因此可能参与这些动物LH分泌的调节。
