Tiensiwakul P
Department of Medical Technology, Chulalongkorn University, Bangkok, Thailand.
Clin Lab Sci. 1990 Sep-Oct;3(5):341-3.
A solid-phase hemadsorption assay (SPHA) for Treponema pallidum was evaluated using serum and umbilical cord blood. A total of 133 sera reactive using immunofluorescent antibody assay were tested by SPHA for IgM antibody against T. pallidum. They were categorized into clinical stages of syphilis, including eight sera with primary, 32 sera with secondary, 68 sera with latent, 21 sera with unclassified, and four sera with congenital syphilis. Sera were reactive for 75%, 90.6%, and 33.8% using SPHA in the primary, secondary, and latent phases, respectively. The method gave 57.1% reactivity for the unclassified phase of syphilitic sera and 100% reactive for the congenital syphilitic sera. All 80 immunofluorescent nonreactive sera were nonreactive by SPHA. Of 19 reactive cord blood samples tested, seven samples were reactive by SPHA. However, all 20 nonreactive umbilical cord blood samples were nonreactive by SPHA. The SPHA assay for the determination of IgM antibody to T. pallidum in serum appears to be sensitive for primary, secondary, and congenital syphilis. The SPHA assay in the cord blood is sensitive and specific.
使用血清和脐带血对梅毒螺旋体的固相血细胞吸附试验（SPHA）进行了评估。对总共133份使用免疫荧光抗体试验呈反应性的血清进行了SPHA检测，以检测抗梅毒螺旋体的IgM抗体。这些血清被分为梅毒的临床阶段，包括8份一期梅毒血清、32份二期梅毒血清、68份潜伏梅毒血清、21份未分类梅毒血清和4份先天性梅毒血清。使用SPHA检测时，一期、二期和潜伏梅毒血清的反应率分别为75%、90.6%和33.8%。该方法对未分类梅毒血清的反应率为57.1%，对先天性梅毒血清的反应率为100%。所有80份免疫荧光检测无反应性的血清，经SPHA检测也无反应性。在检测的19份有反应性的脐带血样本中，有7份样本经SPHA检测呈反应性。然而，所有20份无反应性的脐带血样本经SPHA检测均无反应性。用于测定血清中抗梅毒螺旋体IgM抗体的SPHA试验对一期、二期和先天性梅毒似乎具有敏感性。脐带血中的SPHA试验具有敏感性和特异性。
