枯草芽孢杆菌琥珀酸脱氢酶缺陷型突变体的遗传和生化特性
Genetic and biochemical characterization of mutants of Bacillus subtilis defective in succinate dehydrogenase.
作者信息
Ohné M, Rutberg B, Hoch J A
出版信息
J Bacteriol. 1973 Sep;115(3):738-45. doi: 10.1128/jb.115.3.738-745.1973.
Abstract
Eleven succinate-accumulating mutants of Bacillus subtilis have been mapped by transformation and transduction crosses and characterized with respect to activities of citric acid cycle enzymes. These mutants could be divided into three genetic groups. Nine of the mutants were found to map between argA and leu in the citF locus. A second group was located between lys-1 and trpC2 and the third group could not be located on the B. subtilis chromosome in extensive transduction crosses. All of the citF mutants lack detectable succinate dehydrogenase activity, whereas both of the other groups show a reduced level of this enzyme. In addition, most of the mutants in the citF locus lack cytochrome a, whereas the level of this cytochrome is normal in the other two groups. A procedure has been devised for the solubilization of the succinate dehydrogenase from the membrane of B. subtilis with the non-ionic detergent Brij 58. Some properties of the soluble and bound forms of succinate dehydrogenase are described.
摘要
已通过转化和转导杂交对11株枯草芽孢杆菌琥珀酸积累突变体进行了定位，并对柠檬酸循环酶的活性进行了表征。这些突变体可分为三个遗传组。其中9个突变体被发现在citF基因座中位于argA和leu之间。第二组位于lys-1和trpC2之间，第三组在广泛的转导杂交中无法定位在枯草芽孢杆菌染色体上。所有citF突变体均缺乏可检测到的琥珀酸脱氢酶活性，而其他两组的该酶水平均降低。此外，citF基因座中的大多数突变体缺乏细胞色素a，而在其他两组中该细胞色素的水平正常。已设计出一种用非离子去污剂Brij 58从枯草芽孢杆菌膜中溶解琥珀酸脱氢酶的方法。描述了琥珀酸脱氢酶可溶形式和结合形式的一些特性。
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