Nevasaari K, Alakare B, Kärki N T
Arch Int Pharmacodyn Ther. 1976 Nov;224(1):13-20.
The effect of acetylcholine (ACh) on bile flow and biliary excretion of 3H-ouabain was studied in the physostigmine pretreated isolated perfused rat liver. ACh added into the perfusion medium to give a concentration of 1 X 10(-4)M after the addition of physostigmine (2 X 1 X 10(-5)M) transiently decreased bile flow and biliary concentration of tritium, originated from 3H-ouabain. A deficit of 34 mul of bile during the decreased bile flow could be calculated, compared to the control group. This deficit in bile flow possibly reflects an inhibitory secretory effect of ACh on canalicular bile secretion, or enhanced reabsorption of water in the biliary tree. The decline in the concentration of 3H-oubain in the bile after the addition of ACh indicates that ACh inhibits biliary transport of ouabain by an unknown mechanism, in addition to its effect on bile flow. Physostigmine alone possibly was without an effect on biliary elimination of ouabain. The results show that ACh retards the biliary elimination of ouabain through its influence on bile flow and biliary concentraton of ouabain.
在毒扁豆碱预处理的离体灌注大鼠肝脏中，研究了乙酰胆碱（ACh）对胆汁流量和3H-哇巴因胆汁排泄的影响。在加入毒扁豆碱（2×1×10⁻⁵M）后，向灌注介质中加入ACh使其浓度达到1×10⁻⁴M，可使源于3H-哇巴因的胆汁流量和胆汁中氚的浓度暂时降低。与对照组相比，胆汁流量减少期间可计算出胆汁缺乏34微升。胆汁流量的这种缺乏可能反映了ACh对胆小管胆汁分泌的抑制性分泌作用，或胆管树中水重吸收的增强。加入ACh后胆汁中3H-哇巴因浓度的下降表明，ACh除了对胆汁流量有影响外，还通过未知机制抑制哇巴因的胆汁转运。单独使用毒扁豆碱可能对哇巴因的胆汁消除没有影响。结果表明，ACh通过影响胆汁流量和胆汁中哇巴因的浓度，延缓了哇巴因的胆汁消除。
