Nevasaari K, Alakare B, Kärki N T
Naunyn Schmiedebergs Arch Pharmacol. 1976 Nov;295(2):165-9. doi: 10.1007/BF00499450.
The effects of acetylcholine (ACh). physostigmine, and atropine on bile flow and biliary elimination of digoxin were investigated using isolated rat liver perfusion. 1. ACh in the presence of physostigmine caused a temporary reduction in the bile secretion, while physostigmine alone had no effect on the bile flow. 2. The biliary concentration of radioactivity derived from 3H-digoxin was slightly decreased after an addition of physostigmine alone. This effect of physostigmine was not potentiated by ACh. 3. The addition of ACh decreased transiently the biliary elimination of digoxin, as a result of the reduced bile flow. 4. Atropine in the concentration range of 10(-6)-10(-5) M in the perfusion medium did not affect bile flow or biliary excretion of digoxin; repeated addition of atropine (2 X 2 X 10(-4) M) caused a choleresis lasting over the perfusion period. 5. This choleresis induced by atropine was associated with decreased concentration of tritium in the bile but slightly increased biliary elimination of total radioactivity. 6. The results allow us to draw the conclusion that ACh in the presence of physostigmine has an inhibitory action on bile flow and biliary elimination of digoxin in the isolated perfused rat liver.
采用离体大鼠肝脏灌注法，研究了乙酰胆碱（ACh）、毒扁豆碱和阿托品对胆汁流量及地高辛经胆汁排泄的影响。1. 在毒扁豆碱存在的情况下，ACh可使胆汁分泌暂时减少，而单独使用毒扁豆碱对胆汁流量无影响。2. 单独加入毒扁豆碱后，源自3H-地高辛的放射性在胆汁中的浓度略有下降。毒扁豆碱的这种作用未被ACh增强。3. 由于胆汁流量减少，加入ACh可使地高辛经胆汁的排泄暂时减少。4. 灌注液中毒扁豆碱浓度在10(-6)-10(-5) M范围内时，不影响胆汁流量或地高辛经胆汁的排泄；重复加入阿托品（2×2×10(-4) M）可引起整个灌注期持续的利胆作用。5. 阿托品引起的这种利胆作用与胆汁中氚浓度降低有关，但总放射性经胆汁的排泄略有增加。6. 这些结果使我们得出结论：在毒扁豆碱存在的情况下，ACh对离体灌注大鼠肝脏的胆汁流量及地高辛经胆汁的排泄具有抑制作用。
