Lauer E, Kandler O
Arch Microbiol. 1976 Nov 2;110(23):271-7. doi: 10.1007/BF00690238.
It is demonstrated that most strains of bifidobacteria form much more acetate and less lactic acid from glucose than is to be expected according to the breakdown of glucose via the "bifidoshunt". The analysis of isotope distribution among the fermentation products of glucose labeled in different positions showed that the excess of acetate is the result of the phosphoroclastic splitting of a part of the pyruvate arising from carbons 4, 5, and 6 of glucose. In addition to acetate (carbons 5 and 6), formate is formed from carbon 4 and some acetate is reduced to ethanol. The formation of "extra" acetate occurs mainly during the log phase and is less pronounced in resting cells. The extent of the phosphoroclastic splitting of pyruvate varies considerably among different strains even among those from the same species.
结果表明，与通过“双歧途径”分解葡萄糖所预期的情况相比，大多数双歧杆菌菌株从葡萄糖中产生的乙酸盐要多得多，而乳酸则少得多。对在不同位置标记的葡萄糖发酵产物中同位素分布的分析表明，乙酸盐的过量是由葡萄糖碳4、5和6产生的一部分丙酮酸的磷酸裂解所致。除了乙酸盐（碳5和6）外，碳4还形成甲酸盐，并且一些乙酸盐被还原为乙醇。“额外”乙酸盐的形成主要发生在对数期，在静止细胞中则不太明显。丙酮酸的磷酸裂解程度在不同菌株之间差异很大，即使是同一物种的菌株也是如此。
