Tsuchiya T, Ikarashi Y, Nakamura A
Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
J Long Term Eff Med Implants. 1995;5(4):243-52.
The inhibitory activities on the intercellular gap-junctional communication were investigated using the V79 metabolic cooperation (MC) assay for the detection of tumor-promoting activities of additives such as pigments and phenolic antioxidants. Among six pigments, four chemicals showed inhibitory activities. The inhibitory potencies were ranked in the following order: sudan I > purple 201 > blue 204 > green 202. Sudan I and purple 201 showed stronger inhibitory activities than lithocholic acid, which is known to be a tumor promotor. However, quinizarine and red 225 did not inhibit at any concentration. Relating to eight phenolic antioxidants, four chemicals also showed inhibitory activities. Combining the present findings with previous ones, there are many factors that have tumor-promoting activities via inhibitory action on gap-junctional intercellular communication in biomaterials.
使用V79代谢合作（MC）试验研究了对细胞间缝隙连接通讯的抑制活性，以检测色素和酚类抗氧化剂等添加剂的促肿瘤活性。在六种色素中，有四种化学物质表现出抑制活性。抑制效力按以下顺序排列：苏丹红I＞紫色201＞蓝色204＞绿色202。苏丹红I和紫色201表现出比石胆酸更强的抑制活性，已知石胆酸是一种肿瘤促进剂。然而，醌茜和红色225在任何浓度下均无抑制作用。关于八种酚类抗氧化剂，也有四种化学物质表现出抑制活性。将目前的研究结果与以前的结果相结合，有许多因素通过对生物材料中缝隙连接细胞间通讯的抑制作用而具有促肿瘤活性。
