Bohrman J S, Burg J R, Elmore E, Gulati D K, Barkfnecht T R, Niemeier R W, Dames B L, Toraason M, Langenbach R
Centers for Disease Control, Division of Biomedical and Behavioral Science, Cincinnati, OH 45226.
Environ Mol Mutagen. 1988;12(1):33-51. doi: 10.1002/em.2860120107.
Three laboratories participated in an interlaboratory study to evaluate the usefulness of the Chinese hamster V79 cell metabolic cooperation assay to predict the tumor-promoting activity of selected chemicals. Twenty-three chemicals of different chemical structures (phorbol esters, barbiturates, phenols, artificial sweeteners, alkanes, and peroxides) were chosen for testing based on in vivo promotion activities, as reported in the literature. Assay protocols and materials were standardized, and the chemicals were coded to facilitate unbiased evaluation. A chemical was tested only once in each laboratory, with one of the three laboratories testing only 15 out of 23 chemicals. Dunnett's test was used for statistical analysis, and differences between treated- and control-cell responses were analyzed at P less than or equal to .01. Chemicals were scored as positive (at least two concentration levels statistically different than control), equivocal (only one concentration statistically different), or negative. For 15 chemicals tested in all three laboratories, there was complete agreement among the laboratories for nine chemicals. For the 23 chemicals tested in only two laboratories, there was agreement on 16 chemicals. With the exception of the peroxides and alkanes, the metabolic cooperation data were in general agreement with in vivo data. However, an overall evaluation of the V79 cell system for predicting in vivo promotion activity was difficult because of the organ specificity of certain chemicals and/or the limited number of adequately tested nonpromoting chemicals.
三个实验室参与了一项实验室间研究，以评估中国仓鼠V79细胞代谢合作试验在预测特定化学物质促癌活性方面的实用性。根据文献报道的体内促癌活性，选择了23种化学结构不同的化学物质（佛波酯、巴比妥酸盐、酚类、人工甜味剂、烷烃和过氧化物）进行测试。检测方案和材料进行了标准化，对化学物质进行编码以利于无偏评估。每种化学物质在每个实验室只测试一次，三个实验室中的一个实验室只测试了23种化学物质中的15种。采用邓尼特检验进行统计分析，在P≤0.01时分析处理细胞和对照细胞反应之间的差异。化学物质被评为阳性（至少两个浓度水平与对照有统计学差异）、可疑（只有一个浓度有统计学差异）或阴性。在所有三个实验室测试的15种化学物质中，各实验室对9种化学物质的评价完全一致。在仅在两个实验室测试的23种化学物质中，对16种化学物质的评价一致。除过氧化物和烷烃外，代谢合作数据与体内数据总体一致。然而，由于某些化学物质的器官特异性和/或充分测试的非促癌化学物质数量有限，很难对V79细胞系统预测体内促癌活性进行全面评估。
