Ikawa S, Shibata T, Ando T
J Biochem. 1976 Dec;80(6):1457-60. doi: 10.1093/oxfordjournals.jbchem.a131422.
A new site-specific endonuclease (DNase) was isolated from the cells of Bacillus pumilus AHU 1387 strain. This enzyme (endonuclease R.Bpu 1387) introduced double-stranded scissions at unique sites on DNA's of coli phage lambda, lambdadvl, coli phage T7, Bacillus phage phi105C, Bacillus phage SP10, and Simian Virus 40, in the presence of magnesium ion. The activity was stimulated by the presence of NaCl.
从短小芽孢杆菌AHU 1387菌株的细胞中分离出一种新的位点特异性内切核酸酶（脱氧核糖核酸酶）。这种酶（内切核酸酶R.Bpu 1387）在镁离子存在的情况下，能在大肠杆菌噬菌体λ、λdv1、大肠杆菌噬菌体T7、芽孢杆菌噬菌体phi105C、芽孢杆菌噬菌体SP10和猿猴病毒40的DNA特定位点引入双链切口。氯化钠的存在会刺激该酶的活性。
