Shibata T, Ando T
Biochim Biophys Acta. 1976 Aug 18;442(2):184-96. doi: 10.1016/0005-2787(76)90489-5.
Two species of restriction endonuclease were isolated by gel filtration and DEAE-cellulose chromatography from a cell-free extract of Bacillus amyloliquefaciens (B. subtilits) N strain; a lower molecular weight endonuclease (endonuclease R.BamNI) and a higher molecular-weight one (endonuclease R.BamNx). Both of them required only Mg2+ for their activities. Endonuclease R.BamNx introduced a larger number of site-specific scissions in Excherchia coli phage lambda DNA that endonuclease R.BamNI did. Endonuclease R.BamNx cleaved Bacillus phage phi 105C DNA at the specific sites which are classified into two groups: one type of sites is modified by B. amyloliquefaciens H strain in vivo while the other is not affected. It was also active on DNA'S OF E. coli phage T7, lambdadvl, Simian virus 40 (SV40) and colicinogenic factor ColEI and was inactive on DNAs of Bacillus phages phi 29 and M2. Endonuclease R.BamHI isolated from H strain by Wilson and Young. This endonuclease was active on DNAs of phage lambda, lambdadvl and SV40, adn was inactive on DNAs of phages phi 105C, phi 29, M2 and T7, and ColEI DNA.
通过凝胶过滤和DEAE - 纤维素色谱法从解淀粉芽孢杆菌（枯草芽孢杆菌）N菌株的无细胞提取物中分离出两种限制性内切酶；一种分子量较低的内切酶（内切酶R.BamNI）和一种分子量较高的内切酶（内切酶R.BamNx）。它们的活性都仅需Mg2 + 。内切酶R.BamNx在大肠杆菌噬菌体λDNA中引入的位点特异性切割比内切酶R.BamNI多。内切酶R.BamNx在芽孢杆菌噬菌体phi 105C DNA的特定位点切割，这些位点分为两组：一类位点在体内被解淀粉芽孢杆菌H菌株修饰，而另一类则不受影响。它对大肠杆菌噬菌体T7、lambdadvl、猴病毒40（SV40）和产大肠杆菌素因子ColEI的DNA也有活性，而对芽孢杆菌噬菌体phi 29和M2的DNA无活性。内切酶R.BamHI由威尔逊和杨从H菌株中分离得到。这种内切酶对噬菌体λ、lambdadvl和SV40的DNA有活性，而对噬菌体phi 105C、phi 29、M2和T7的DNA以及ColEI DNA无活性。
