Esmaeli-Gutstein B, Hewlett B R, Pashby R C, Oestreicher J, Harvey J T
Department of Ophthalmology, McMaster University, Hamilton, Ontario, Canada.
Ophthalmic Plast Reconstr Surg. 1999 Mar;15(2):92-9. doi: 10.1097/00002341-199903000-00005.
To identify adrenergic receptor subtypes and their relative distribution in the retractor muscles of the upper eyelid, the levator palpebrae superioris, and the Müller muscle. The pattern of distribution of these receptors in the Müller muscle was further compared in patients with dysthyroid eyelid retraction and in normal subjects.
Müller muscle specimens were collected from 19 patients undergoing ptosis correction and from 8 patients undergoing repair of dysthyroid eyelid retraction. Immunohistochemical staining for alpha 1-, alpha 2-, beta 1-, and beta 2-adrenergic receptors was performed using antihuman rabbit polyclonal antibodies.
alpha 2-Adrenergic receptors were the predominant subtype in the Müller muscle, and beta 1-adrenergic receptors were the predominant subtype in the levator muscle. There was no significant difference in the staining pattern between specimens collected from patients with dysthyroid eyelid retraction and those from normal subjects.
The interaction between the alpha 2 and beta 1 receptors in the upper eyelid retractor muscles may be important in the control of the upper eyelid position and may contribute to the development of dysthyroid eyelid retraction. Specific alpha 2 antagonists could be developed and may be effective pharmacologic agents for the treatment of eyelid retraction.
确定肾上腺素能受体亚型及其在上睑提肌、上睑退缩肌和米勒肌中的相对分布。并对甲状腺功能异常性上睑退缩患者和正常受试者米勒肌中这些受体的分布模式进行进一步比较。
从19例接受上睑下垂矫正手术的患者和8例接受甲状腺功能异常性上睑退缩修复手术的患者中采集米勒肌标本。使用抗人兔多克隆抗体对α1、α2、β1和β2肾上腺素能受体进行免疫组织化学染色。
α2肾上腺素能受体是米勒肌中的主要亚型，β1肾上腺素能受体是提上睑肌中的主要亚型。甲状腺功能异常性上睑退缩患者和正常受试者的标本染色模式无显著差异。
上睑退缩肌中α2和β1受体之间的相互作用可能对上睑位置的控制很重要，并可能导致甲状腺功能异常性上睑退缩的发生。可以开发特异性α2拮抗剂，其可能是治疗上睑退缩的有效药物。
