Cameron R A, Leahy P S, Britten R J, Davidson E H
Division of Biology 156-29, California Institute of Technology, Pasadena, California, 91125, USA.
Dev Biol. 1999 Apr 15;208(2):255-64. doi: 10.1006/dbio.1999.9224.
Strongylocentrotus purpuratus, a major research model in developmental molecular biology, has been inbred through six generations of sibling matings. Though viability initially decreased, as described earlier, the inbred line now consists of healthy, fertile animals. These are intended to serve as a genomic resource in which the level of polymorphism is decreased with respect to wild S. purpuratus. To genotype the inbred animals eight simple sequence genomic repeats were isolated, in context, and PCR primers were generated against the flanking single-copy sequences. Distribution and polymorphism of these regions of the genome were studied in the genomes of 27 wild individuals and in a sample of the inbred animals at F2 and F3 generations. All eight regions were polymorphic, though to different extents, and their homozygosity was increased by inbreeding as expected. The eight markers suffice to identify unambiguously the cellular DNA of any wild or F3 S. purpuratus individual.
紫海胆（Strongylocentrotus purpuratus）是发育分子生物学中的一种主要研究模型，已经通过六代同胞交配进行了近亲繁殖。如前所述，虽然最初的活力有所下降，但现在的近交系由健康、可育的动物组成。这些动物旨在作为一种基因组资源，其中多态性水平相对于野生紫海胆有所降低。为了对近交动物进行基因分型，从上下文中分离出了八个简单序列基因组重复序列，并针对侧翼单拷贝序列生成了PCR引物。在27个野生个体的基因组以及F2和F3代近交动物样本中研究了基因组这些区域的分布和多态性。所有八个区域都具有多态性，尽管程度不同，并且正如预期的那样，近亲繁殖增加了它们的纯合性。这八个标记足以明确识别任何野生或F3代紫海胆个体的细胞DNA。
