Di Salvo C, Sambataro S, De Meo S
Quad Sclavo Diagn. 1976 Mar;12(1):90-103.
A single, rapid, precise and automatizzable micro-method for determining serum bilirubin has been described. The method requires a little while of serum. It is possible reader the bilirubin concentration at last of 30 mg/100 ml without of serum dilution. The determination of "direct bilirubin" has been examined in connection with the reaction time. The reliability of the our diazo-method has been established by comparison with well-known diazo-methods and by demonstrating the effect of hemolysis.
已描述了一种用于测定血清胆红素的单一、快速、精确且可自动化的微量方法。该方法所需血清量少。无需稀释血清即可读取高达30mg/100ml的胆红素浓度。结合反应时间对“直接胆红素”的测定进行了研究。通过与知名重氮法比较并证明溶血的影响,确立了我们重氮法的可靠性。