Lwaleed B A, Bass P S, Francis J L, Chisholm M
University Department of Haematology, Southampton University Hospitals, UK.
Nephrol Dial Transplant. 1999 Mar;14(3):588-96. doi: 10.1093/ndt/14.3.588.
We have previously explored the clinical significance of urinary tissue factor (uTF) in patients with glomerulonephritis (GN) and malignancy. However, the functional and structural properties and putative cell of origin of uTF are poorly documented. In these studies we investigate these aspects of uTF.
Functional and structural properties of uTF were investigated using a one stage kinetic chromogenic assay, an enzyme-linked immunoabsorbent assay (ELISA) and transmission electron microscopy (TEM) on urine samples collected from healthy controls (n=69). The distribution of uTF and anionic phospholipid in the kidney were studied in sections from normal areas of nephrectomy specimens, using an immunoperoxidase technique. These were stained for tissue factor (TF) antigen and recombinant Annexin V.
We found uTF to be present on subcellular particles as visualized by TEM. These particles contained anionic phospholipid as evidenced by binding to Annexin V fluorescein isothiocyanate (FITC). Although TF is present in urine in a functional and antigenic form no association was observed between the two. Using immunoperoxidase-based techniques, the cytoplasm of both distal and proximal tubules (but not glomerular cells) was positive for TF antigen and Annexin V.
uTF is found on subcellular particles which provide lipid for its functional activity. Both uTF and its associated vesicles are found in the renal tubular cells.
我们之前探讨了尿组织因子（uTF）在肾小球肾炎（GN）和恶性肿瘤患者中的临床意义。然而，uTF的功能和结构特性以及假定的起源细胞却鲜有文献记载。在这些研究中，我们对uTF的这些方面进行了调查。
使用一步动力学显色测定法、酶联免疫吸附测定法（ELISA）和透射电子显微镜（TEM）对从健康对照者（n = 69）收集的尿液样本进行uTF的功能和结构特性研究。采用免疫过氧化物酶技术，在肾切除标本正常区域的切片中研究uTF和阴离子磷脂在肾脏中的分布。这些切片用组织因子（TF）抗原和重组膜联蛋白V进行染色。
通过TEM观察发现uTF存在于亚细胞颗粒上。这些颗粒含有阴离子磷脂，与异硫氰酸荧光素（FITC）标记的膜联蛋白V结合可证明这一点。尽管TF以功能和抗原形式存在于尿液中，但两者之间未观察到关联。使用基于免疫过氧化物酶的技术，远端和近端肾小管（而非肾小球细胞）的细胞质中TF抗原和膜联蛋白V呈阳性。
uTF存在于为其功能活性提供脂质的亚细胞颗粒上。uTF及其相关囊泡均存在于肾小管细胞中。
