肾小球肾炎中的尿组织因子：肾小球损伤的潜在标志物？

Urinary tissue factor in glomerulonephritis: a potential marker of glomerular injury?

作者信息

Lwaleed B A, Bass P S, Chisholm M, Francis J L

机构信息

Southampton University Hospitals, United Kingdom.

出版信息

J Clin Pathol. 1997 Apr;50(4):336-40. doi: 10.1136/jcp.50.4.336.

DOI:10.1136/jcp.50.4.336

PMID:9215153

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499887/

Abstract

AIM

To investigate the significance of urinary tissue factor (uTF) concentrations in patients with glomerulonephritis.

METHODS

Urine samples were collected from normal subjects (n = 57), patients with uncomplicated renal stones (n = 30), and patients with glomerulonephritis (n = 150). Samples were then centrifuged and the pellets solubilised in n-octyl-beta-glucopyranoside. uTF concentrations were determined using a one stage kinetic chromogenic assay.

RESULTS

The uTF concentration was higher in patients with glomerulonephritis than in normal controls (p < 0.01) or in patients with renal stones (p < 0.05). uTF activity correlated with the protein creatinine index (PCI, r = 0.41, p < 0.001) and seven patients with glomerulonephritis and a PCI < or = 0.1 g/mmol had raised uTF. Glomerulonephritis patients were subdivided into two groups depending on the PCI: < 0.2 g/mmol creatinine (mild to moderate proteinuria, group I) and > or = 0.2 g/mmol creatinine (heavy proteinuria, group II). In group I, uTF concentrations were higher in patients with either immune complex (IC) glomerulonephritis (p < 0.01) or non-IC (p < 0.05) glomerulonephritis than in normal controls. In group II, the IC glomerulonephritis group had higher uTF concentrations than normal controls (p < 0.001) or patients with renal stones (p < 0.01); and non-IC glomerulonephritis patients had higher uTF than normal controls (p < 0.01). When the glomerulonephritis groups were divided into broad WHO subtypes, the significance level varied with the type of glomerulonephritis.

CONCLUSIONS

uTF is increased in patients with glomerulonephritis, and its concentration may reflect the aetiopathogenesis of glomerulonephritis.

摘要

目的

探讨肾小球肾炎患者尿组织因子（uTF）浓度的意义。

方法

收集正常受试者（n = 57）、单纯肾结石患者（n = 30）和肾小球肾炎患者（n = 150）的尿液样本。然后将样本离心，沉淀物用正辛基-β-D-葡萄糖苷溶解。使用一步动力学显色法测定uTF浓度。

结果

肾小球肾炎患者的uTF浓度高于正常对照组（p < 0.01）或肾结石患者（p < 0.05）。uTF活性与蛋白肌酐指数（PCI，r = 0.41，p < 0.001）相关，7例肾小球肾炎且PCI≤0.1 g/mmol的患者uTF升高。根据PCI将肾小球肾炎患者分为两组：肌酐<0.2 g/mmol（轻度至中度蛋白尿，I组）和≥0.2 g/mmol肌酐（重度蛋白尿，II组）。在I组中，免疫复合物（IC）肾小球肾炎患者（p < 0.01）或非IC肾小球肾炎患者（p < 0.05）的uTF浓度高于正常对照组。在II组中，IC肾小球肾炎组的uTF浓度高于正常对照组（p < 0.001）或肾结石患者（p < 0.01）；非IC肾小球肾炎患者的uTF高于正常对照组（p < 0.01）。当将肾小球肾炎组分为世界卫生组织的大致亚型时，显著性水平因肾小球肾炎类型而异。