Ohbayashi E, Matsushima K, Hosoya S, Abiko Y, Yamazaki M
Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
J Endod. 1999 Jan;25(1):30-3. doi: 10.1016/S0099-2399(99)80395-1.
The purpose of the present study was to investigate the effect of laser irradiation on calcified nodule formation in human dental pulp (HDP) cells. HDP cells were irradiated once with a Ga-Al-As laser for 5 and 10 min, and calcified nodule formation was determined by von Kossa staining. The laser irradiation increased the number of calcified nodules in a time-dependent manner. The activity of alkaline phosphatase and production of collagen and osteocalcin in conditioned medium were measured. Both were higher in the irradiated group than in the nonirradiated group. These results suggested that formation of calcified nodules in HDP cells, as well as in alkaline phosphatase activity, the production of collagen and osteocalcin were enhanced by laser irradiation.
本研究的目的是调查激光照射对人牙髓（HDP）细胞中钙化结节形成的影响。用Ga-Al-As激光对HDP细胞照射一次，照射时间分别为5分钟和10分钟，通过冯·科萨染色法测定钙化结节的形成情况。激光照射使钙化结节的数量呈时间依赖性增加。检测了条件培养基中碱性磷酸酶的活性以及胶原蛋白和骨钙素的生成量。照射组的这两项指标均高于未照射组。这些结果表明，激光照射可增强HDP细胞中钙化结节的形成，以及碱性磷酸酶活性、胶原蛋白和骨钙素的生成。
