Dental Research Center, Mashhad University of Medical Sciences, P.O. Box: 91735-984, Vakilabad Blvd, Mashhad, Iran.
Department of Endodontics, Faculty of Dentistry, Golestan University of Medical Sciences, Gorgan, Iran.
Lasers Med Sci. 2021 Aug;36(6):1289-1295. doi: 10.1007/s10103-020-03239-0. Epub 2021 Jan 18.
Blood extracts containing platelet products are gaining popularity in promoting healing and pulp regeneration. This study was designed to evaluate the effect of platelet-rich plasma (PRP) and gallium-aluminum-arsenide (GaAlAs) laser on proliferation and differentiation of human dental pulp stem cells (hDPSCs). In this ex vivo study, hDPSCs isolated from impacted mandibular third molars were cultured in Dulbecco's Modified Eagle's medium )DMEM(with 10% fetal bovine serum (FBS). After reaching the desired confluence, the cells were distributed into 4 groups, namely, control, PRP, laser, and PRP+laser for MTT assay and alkaline phosphatase (ALP) test. In the PRP and PRP+laser groups, 10% PRP was added to each well on the plate. In the laser and PRP+laser groups, as for the proliferation test, laser irradiation was carried out for 45 s, while 135 s was designated for ALP test. After 1, 3, and 5 days, cell proliferation and ALP activity were assessed using MTT and ALP colorimetric assay, respectively. Two-way ANOVA was utilized to analyze data. In PRP and PRP+laser groups, cell proliferation and viability increased until day 3 but began to decline afterwards until the 5th day. In the laser group, the increase in proliferation and viability was observed till day 5 which was less than the control group. Laser and control groups exhibited significantly higher cell viability and proliferation than both PRP and PRP+laser groups. ALP activity was more pronounced in PRP+laser, PRP, and laser in descending order; however, all were less than that of the control group. Only in the control group did the ALP activity augment during the 5-day period. Laser irradiation could induce pulp cell proliferation and demonstrated a better performance than PRP in this regard.
富含血小板的血液提取物在促进愈合和牙髓再生方面越来越受欢迎。本研究旨在评估富血小板血浆（PRP）和砷化镓铝（GaAlAs）激光对人牙髓干细胞（hDPSCs）增殖和分化的影响。在这项离体研究中，从下颌第三磨牙中分离出人牙髓干细胞，在含有 10%胎牛血清（FBS）的 Dulbecco 改良 Eagle 培养基（DMEM）中培养。达到所需的汇合度后，将细胞分为 4 组，即对照组、PRP 组、激光组和 PRP+激光组，用于 MTT 测定和碱性磷酸酶（ALP）试验。在 PRP 和 PRP+激光组中，向每个孔中加入 10%的 PRP。在激光和 PRP+激光组中，对于增殖试验，进行 45 s 的激光照射，而 ALP 试验则指定 135 s。在第 1、3 和 5 天，使用 MTT 和 ALP 比色法分别评估细胞增殖和 ALP 活性。采用双因素方差分析进行数据分析。在 PRP 和 PRP+激光组中，细胞增殖和活力在第 3 天增加，但随后开始下降，直到第 5 天。在激光组中，观察到增殖和活力的增加持续到第 5 天，低于对照组。激光和对照组的细胞活力和增殖均显著高于 PRP 和 PRP+激光组。ALP 活性按 PRP+激光、PRP 和激光的顺序逐渐降低，但均低于对照组。只有在对照组中，ALP 活性在 5 天内增加。激光照射可以诱导牙髓细胞增殖，在这方面的表现优于 PRP。
